PickMutant™ DNA Shuffling Kit
Efficient DNA shuffling kit for homologous recombination and mutant library generation.
The PickMutant™ DNA Shuffling Kit enables reliable and efficient mutagenesis through DNA shuffling, a homologous recombination method pioneered by Stemmer. DNA shuffling involves DNase I digestion of target genes into random fragments, followed by reassembly through primerless PCR.
This process generates libraries of recombinant genes containing crossovers and new point mutations (mutation rate ≈0.7%), providing a powerful tool for protein engineering, enzyme optimization, and molecular evolution studies.
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Price: 334.80 €
Detailed information:
Advantages & Features
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Efficient: proven homologous recombination method.
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Reliable: reproducible results.
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Robust: optimized DNase I digestion and reassembly.
Specifications
Includes
– 40 μL Taq Polymerase (5 U/μL)
– 500 μL PCR Buffer (10x)
– 80 μL dNTP Mix (10 mM)
– 100 μL DNase I Solution (0.1 U/ μL)
– 200 μL Reaction Buffer (10x)
– 100 μL Stop Solution
– 3 x 1.5 mL PCR-grade Water
Download documentation
Datasheet MSDSApplications
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Generation of mutant gene libraries.
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Homologous recombination of genes.
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In vitro molecular evolution.
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Recombination of sequence blocks.
Tables & Figures
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Quality Control
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Tested with control DNA insert provided.
Advice
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Avoid repeated freeze-thaw cycles.
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Handle DNase I carefully and protect from heat.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
Citations
Wong, T. S., & Tee, K. L. (2020). Continuing from Protein Variants. In A Practical Guide to Protein Engineering (pp. 187-195). Springer, Cham.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
DNA shuffling kit, Directed evolution DNA kit, Recombination mutagenesis kit, DNA recombination kit, Molecular evolution kit
Also known as:
- Spanish: Kit de recombinación de ADN, Kit de evolución dirigida, Kit de DNA shuffling
- French: Kit de recombinaison ADN, Kit d’évolution dirigée, Kit de DNA shuffling
- German: DNA-Shuffling-Kit, Kit für gerichtete Evolution, DNA-Rekombinationskit
- Italian: Kit di ricombinazione del DNA, Kit evoluzione diretta, Kit DNA shuffling
FAQs
Q: What is the typical mutation rate of DNA shuffling?
A: Around 0.7% with additional recombination crossovers.
Q: What makes DNA shuffling different from error-prone PCR?
A: DNA shuffling combines recombination with point mutation, while epPCR introduces random misincorporation only.
Q: What are the main applications?
A: Protein engineering, enzyme evolution, synthetic biology.
Marco Rinaldi –
We used this kit for directed evolution experiments combining related gene variants. The workflow was easy to follow and adapted well to our existing cloning procedures. It was useful to reduce hands-on optimization during the library construction step.