PickMutant™ Random Mutagenesis Kit
High-efficiency random mutagenesis kit based on dPTP nucleotide analog incorporation in PCR.
The PickMutant™ Random Mutagenesis Kit enables robust, reliable, and efficient random mutagenesis through incorporation of the nucleotide analog dPTP during PCR. The analog increases substitution frequency, generating diverse mutant libraries essential for protein engineering, directed evolution, and functional studies.
Following incorporation, a second PCR step with natural dNTPs eliminates analog residues, yielding mutation rates of up to 20%. This system provides reproducible transitions (A→G, T→C, G→A, C→T) and is optimized for Taq polymerase. The kit ensures reproducibility and quality required for advanced mutagenesis workflows.
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Price: 215.80 €
1 in stock
Detailed information:
Advantages & Features
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Efficient mutagenesis: up to 20% mutation rate.
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Reliable: consistent results across replicates.
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Robust: compatible with Taq DNA polymerase.
Specifications
Includes
– 40 μL Taq Polymerase (5 U/μL)
– 200 μL PCR Buffer (10x)
– 100 μL dNTP Mix (10 mM)
– 100 μL MgCl2 (25 mM)
– 40 μL dPTP (10 mM)
– 2 x 1 mL PCR-grade Water
Download documentation
Datasheet MSDSApplications
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Random mutagenesis by dPTP analog incorporation.
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Generation of mutant libraries for protein engineering.
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Directed evolution studies.
Tables & Figures
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Quality Control
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Tested using control insert DNA.
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Verified mutation frequency reproducibility.
Advice
- Aliquot reagents to avoid freeze-thaw cycles.
- dPTP is most stable when stored as aqueous solution in the freezer (- 20° C necessary, – 80 °C recommended), however, at Ambient Temperature the compound slowly starts to decompose. Thus, in order to maintain its original high quality, it is recommended to allow thawing only before using the product. If you will not use up the vial with one application, please aliquot the contents of the vial in order to avoid repeated freeze/thaw cycles for the rest. When making such aliquots be sure to operate quickly and to freeze the vial again as soon as possible.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
Random mutagenesis kit, DNA mutagenesis kit, Mutation generation kit, Directed evolution mutagenesis kit, PCR mutagenesis kit
Also known as:
- Spanish: Kit de mutagénesis aleatoria, Kit de mutación de ADN, Kit de evolución dirigida
- French: Kit de mutagénèse aléatoire, Kit de mutation ADN, Kit d’évolution dirigée
- German: Kit für zufällige Mutagenese, DNA-Mutagenesekit, Kit für gerichtete Evolution
- Italian: Kit mutagenesi casuale, Kit mutazione DNA, Kit evoluzione diretta
FAQs
Q: What is the typical mutation rate?
A: Up to 20% depending on PCR conditions.
Q: Which DNA polymerase is recommended?
A: Taq DNA polymerase is optimized for this kit.
Q: What types of mutations are introduced?
A: Transition mutations: A→G, T→C, G→A, C→T.
Ana Lima –
Usamos este kit para gerar bibliotecas de variantes em projetos de engenharia de proteinas. A incorporacao do analogo dPTP durante a PCR permite atingir taxas de mutacao de ate 20%, e a segunda etapa de PCR com dNTPs naturais e eficiente para eliminar os residuos do analogo. Os resultados sao reproduziveis entre experimentos e o protocolo e bem adequado para fluxos de evolucao dirigida.