PickMutant™ Error Prone PCR Kit
Reliable error-prone PCR kit for generating diverse mutant libraries with 0.6–2.0% mutation rates.
The PickMutant™ Error-Prone PCR Kit is a robust tool for generating diverse mutant libraries through controlled misincorporation during PCR. Based on the Cadwell & Joyce protocol, it exploits the low fidelity of Taq DNA polymerase, enhanced by Mn²⁺ and unbalanced dNTP concentrations, achieving mutation rates between 0.6–2.0%.
Ideal for molecular evolution, protein engineering, and directed evolution studies, this kit provides reproducible and reliable random mutagenesis in a convenient ready-to-use format.
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Price: 240.79 €
Detailed information:
Advantages & Features
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Efficient: 0.6–2.0% mutation frequency.
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Reliable & reproducible.
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Robust: optimized for mutant library generation.
Specifications
Includes
– 50 μL Taq Polymerase (5 U/μL)
– 200 μL 10x ep-PCR Buffer
– 80 μL ep-dNTP Mix (unbalanced dNTP ratio)
– 200 μL MnCl2 Solution (25mM)
– 2 x 1.5 mL Water (PCR Grade)
Download documentation
Datasheet MSDSApplications
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Random mutagenesis via error-prone PCR.
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In vitro molecular evolution.
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Protein engineering and directed evolution studies.
Tables & Figures
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Quality Control
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Tested with control insert DNA.
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Verified reproducible mutation frequencies.
Advice
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Mix reagents gently before use.
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Avoid repeated freeze-thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 ºC (NON Frost-Free Freezer) and Avoid freeze/thaw cycles.
Citations
- Khlusevich, Y., Matveev, A., Emelyanova, L., Goncharova, E., Golosova, N., Pereverzev, I., & Tikunova, N. (2022). New p35 (H3L) Epitope Involved in Vaccinia Virus Neutralization and Its Deimmunization. Viruses, 14(6), 1224.
- Wong, T. S., & Tee, K. L. (2020). Gene Mutagenesis. In A Practical Guide to Protein Engineering (pp. 121-148). Springer, Cham.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q: What mutation rate does this kit achieve?
A: Between 0.6–2.0%, depending on PCR conditions.
Q: What makes it different from random mutagenesis with dPTP?
A: Error-prone PCR uses Taq low fidelity with Mn²⁺/dNTP imbalance, not analog incorporation.
Q: What are typical applications?
A: Protein engineering, molecular evolution, directed evolution.
