pSpark® II

For highly Efficient, Accurate & Easy general Cloning with classical MCS, without the use of Toxic Genes

pSpark® II is a highly efficient, accurate and easy-to-use DNA Cloning system based on a breakthrough technology for cloning blunt ended DNA generated by PCR with a proofreading or high fidelity DNA polymerases.

The vector is prepared by digestion of pSpark® II at the EcoRV site before treating both ends to prevent vector self-ligation. The end treatment is supported by a proprietary know-how that guarantees a higher cloning efficiency than with simply a desphorylated vector.

Price: 175.00 

SKU: C0002 Categories: ,

Detailed information:

Advantages & Features
  • Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Great efficiency: over hundreds positive colonies with few nanograms of insert.
  • High stability: eliminates cloning bias or pitfalls.
  • Time-saving protocol: no hidden steps such as phosphorylation, just ligation after PCR and transformation.
  • Powerful: clone from less than 1 ng/kb to up to 14 kb; obtain 4-fold more positive colonies using 3-fold less DNA insert.
  • Easy-to-use: eliminate recombinant screening due to its minumum background (lower than 1%), avoiding “suicide” strategies from toxic genes.
  • Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers.
  • Flexible: ligation time from 10 minutes to overnight.
  • Robust for every DNA size: just 6.7 ng per kb of insert needed for optimal ligation.
  • High cost-saving:  reduces your cloning costs as no expensive phosphorylated primers are needed.
  • Eliminates positive selection vector.

– 20 rxn pSpark® II (20 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)

Download documentation
  • General cloning.
  • Clone PCR fragments included in a low amount.
  • Cloning of PCR products amplified with High Fidelity polymerases.
  • Cloning of PCR fragments generated with a blend of polymerases.
  • Production of ssDNA.
  • Blue/white screening for recombinants.
  • In vitro transcription from T7/SP6 dual-opposed promoters.
Tables & Figures


Quality Control
  • Functional test using a 1.0 kb PCR fragment.
  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.
Storage, Shipping & Guarantee
  • Shipped in: Gel pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
    Cart preview
    Your cart is empty