CVX5a™ Chemically Competent Cells

Versatile, Convenient & Cost-effective solution for routine Subcloning Procedures

CVX5α™ Chemically Competent Cells is a versatile, convenient and cost-effective solution for routine subcloning procedures or any application where the starting DNA is not limiting. It is calcium chloride-treated to facilitate attachment of the plasmid DNA to the competent cell membrane.

CVX5α™ Genotype:  F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).

Price: 138.60 345.00 

SKU: C0031 Categories: ,

Detailed information:

Advantages & Features
  • Versatile: proven performance for high-efficiency transformation in a wide variety of applications.
  • Convenient: ideal for routine.
  • Compatible: with blue/white screening of colonies on bacterial plates containing Bluo-gal or X-gal.

CVX5α™ Genotype:  F – , gyrA96, recA1, endA1, thi1, hsdR17 (rK – mK +), deoR, supE44, ∆ (lacZYA-argF) U169 φ80lacZ∆M15 (similar to DH5™ ).


Includes for 40 rxn:
– 2,000 µL CVX5α™ (1 x 107 CFU/µg)
– 10 µL pUC18 Transformation Control Plasmid (10 ng/µL)
– 50 mL SOC Medium
– Dry ice

Download documentation
  • Routine cloning and subcloning of genes into plasmid vectors.
Tables & Figures

Quality Control
  • Each lot of competent cells is tested to verify transformation efficiencies using 100 pg pUC18 supercoiled DNA and the recommended protocol.
  • Under these conditions, transformation efficiency will be ≥ 1 x 107 cfu/μg pUC18.
  • Transformation efficiency is defined as the number of colony forming units (cfu) produced by transforming 1 μg of plasmid (3 kb) into a given volume of competent cells.
  • Note: Optimal competence for cloning but it is not enough for the generation of cDNA libraries.
Storage, Shipping & Guarantee
  • Shipped in: Dry ice.
  • Storage: Upon receipt, store cells immediately at -80 °C.
  • Recommendations: Storage at -20 °C will result in a significant decrease in transformation efficiency. Please note that competent cells are very sensitive to cycles of freezing and thawing and should not be exposed to temperature variations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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