pSpark® TA

– 20 rxn pSpark® TA (50 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 5 µL Insert Control 600bp control insert

• Cloning of non-proofreading PCR fragments.
• Production of ssDNA.
• Blue/white screening for recombinants.
• In vitro transcription from T7/SP6 dual-opposed promoters.

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• Functional test using a 600 pb PCR fragment.

• Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.

• Shipped in: Gel Pack.
• Storage: -20 ºC (NON Frost-Free Freezer).

• Sanchez-Briñas, A., Duran-Ruiz, C., Astola, A., Arroyo, M. M., Raposo, F. G., Valle, A., & Bolivar, J. (2023). ZNF330/NOA36 interacts with HSPA1 and HSPA8 and modulates cell cycle and proliferation in response to heat shock in HEK293 cells. Biology Direct, 18(1), 1-14.
• Huertas-García, A. B., Guzmán, C., Tabbita, F., & Alvarez, J. B. (2022). Allelic Variation of Puroindolines Genes in Iranian Common Wheat Landraces. Agriculture, 12(8), 1196.
• Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
• Morales de los Ríos Martín, L. (2022). Influencia del transportador de nitrato NRT1. 4/NPF6. 2 en la nutrición de potasio en Arabidopsis thaliana.
• Oliveras, À., Camó, C., Caravaca-Fuentes, P., Moll, L., Riesco-Llach, G., Gil-Caballero, S., … & Planas, M. (2022). Peptide Conjugates Derived from flg15, Pep13, and PIP1 That Are Active against Plant-Pathogenic Bacteria and Trigger Plant Defense Responses. Applied and Environmental Microbiology, e00574-22.
• Álvarez Escribano, I. (2019). Caracterización de los sRNAs reguladores NsrR1 y NsiR3 de la cianobacteria Nostoc sp. PCC 7120.
• Moreno Cabezuelo, J. Á. (2019). Glucose uptake in marine cyanobacteria: regulation, expression of the transporter and effects on the proteome and metabolome.
• Alvarez, J. B., Castellano, L., Recio, R., & Cabrera, A. (2019). Wx Gene in Hordeum chilense: Chromosomal Location and Characterisation of the Allelic Variation in the Two Main Ecotypes of the Species. Agronomy, 9(5), 261.
• Lindo, L., McCormick, S. P., Cardoza, R. E., Kim, H. S., Brown, D. W., Alexander, N. J., … & Gutiérrez, S. (2018). Role of Trichoderma arundinaceum tri10 in regulation of terpene biosynthetic genes and in control of metabolic flux. Fungal Genetics and Biology.
• Huyghe, J. (2017). Selection of reference genes for gene expression studies in Lactic acid bacteria.
• Rivas, A. J., von Hoven, G., Neukirch, C., Meyenburg, M., Qin, Q., Füser, S., … & Husmann, M. (2015). Phobalysin, a Small β-Pore-Forming Toxin of Photobacterium damselae subsp. damselae. Infection and immunity, 83(11), 4335-4348.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.