pSpark® IV

For highly Efficient, Stable & Powerful Cloning under transcription-free conditions

pSpark® IV is a highly efficient, accurate and easy-to-use DNA cloning system that exploit its very low background feature for the expression of toxic genes under transcription-free conditions. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region remains and you can find blue colony). The vector is ideal for cloning genes that produce toxic polypeptides by transcription/ translation.

Price: 184.68 

SKU: C0004 Categories: ,

Detailed information:

Advantages & Features
  • Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Transcription-free.
  • Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%).
  • High stability: eliminates cloning bias or pitfalls.
  • Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
  • Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
  • Great versatility: compatible with any protocol, proofreading Polymerase, Competent Cells, ligation time or primers.
  • Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
  • Cost avoidance: removes expensive primer phosphorylation use.
  • Eliminates positive selection vector.

– 20 rxn pSpark® IV (20 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)

Download documentation
  • Cloning of high fidelity PCR amplified products.
  • Production of ssDNA.
  • In vitro transcription from T7/SP6 dual-opposed promoters.
  • Cloning of toxic genes.
Tables & Figures


Quality Control
  • Functional test using a 1.0 kb PCR fragment.
  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.
Storage, Shipping & Guarantee
  • Shipped in: Gel Pack.
  • Storage: -20º C (NON Frost-Free Freezer).
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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