pMBL™ T Vector

For a Efficient, Convenient & Fast Cloning of DNA fragments with A overhangs

pMBL™ T-Vector DNA Cloning Kit is an efficient, convenient and fast system for the cloning of PCR products. The vector is prepared by cutting pMBL™ T-Vector with EcoRV and adding a 3´ terminal thymidine to both ends. These single 3´-T overhangs at the insertion site greatly improve the efficiency of ligation of a PCR product into the plasmids by preventing recircularization of the vector and providing a compatible overhang for PCR products generated by certain thermostable polymerases such as Horse-Power™ Taq DNA Polymerase.

These Polymerases often add a single deoxyadenosine, in a template-independent fashion, to the 3´-ends of the amplified fragments.

Price: 188.31 

SKU: C0030 Categories: ,

Detailed information:

Advantages & Features
  • Highly efficient: greater than 90% white colonies in a transformation with supplied insert control.
  • Proven performance: more than 1,000 recombinant colonies expected in optimal conditions.
  • Fast and easy protocol: results from 15 min protocol.
  • Optimized: improve efficiency of ligation of a PCR product into the plasmid.
  • Compatible: overhang for ligation of PCR products preventing recircularization of the vector.
  • Designed by cutting the vector with EcoRV and adding a 3´ terminal thymidine to both ends.

– 20 µL pMBL™ T-Vector (50 ng/µL)
– 20 µL T4 DNA Ligase (5U/Weiss)
– 100 µL T4 DNA Ligase Buffer (10x)
– 5 µL Insert Control 600 bp (30 ng/µL)

Download documentation
  • Cloning of PCR fragments into DNA Cloning vector.
  • Blue/white screening for recombinants.
Tables & Figures


Quality Control
  • Functional test using a 600 pb PCR fragment.
Storage, Shipping & Guarantee
  • Shipped in: Gel Pack.
  • Storage: -20 ºC (NON Frost-Free Freezer).
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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