pSpark® V

For highly Efficient, Accurate & Easy Cloning with pBR322 and Transcription-free conditions

pSpark® V is a highly efficient, accurate and easy-to-use DNA cloning system developed with low copy number, as a help for cloning of inserts with the highers kb. This low copy variant is also transcription-free, for the most demanding cloning tasks. In this vector, the lac promoter has been eliminated and therefore blue/white screening is not allowed (alpha-peptide coding region has been truncated).

Price: 184.68 

SKU: C0005 Categories: ,

Detailed information:

Advantages & Features
  • Unprecedented high cloning efficiency: more than 2,500 positive colonies expected under optimal conditions.
  • Transcription-free.
  • Easy-to-use: eliminate screening of recombinants due to its minumum background (lower than 1%).
  • High stability: removes cloning bias or pitfalls.
  • Time-saving protocol: avoids any step required after PCR, just 19 minutes from PCR to plating.
  • Powerful: obtain 5-fold more positive colonies using 10-fold less DNA insert.
  • Great versatility: compatible with any protocol, proofreading polymerase, competent cells, ligation time or primers.
  • Sensitive: clone from 50 bp insert to up to 14 kb with just 5 ng per kb of insert.
  • Optimized: truncated alpha-peptide coding region.
  • Cost avoidance: avoids expensive primer phosphorylation use.
  • Eliminates positive selection vector.
Specifications
Includes

– 20 rxn pSpark® V (20 ng/µL)
– 20 µL T4 DNA Ligase (5 Weiss U/µL)
– 100 µL T4 DNA Ligase Buffer (10x)
– 150 µL PEG 6000 (10x)
– 5 µL Insert Control 1 kb (20 ng/µL)

Download documentation
Datasheet
MSDS
Applications
  • Cloning of toxic genes.
  • Cloning of unstable genes, for example genes with repeated sequences.
  • Cloning of high fidelity PCR amplified products.
  • Production of ssDNA.
  • Blue/white screening for recombinants.
  • In vitro transcription from T7/SP6 dual-opposed promoters.
Tables & Figures

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Quality Control
  • Functional test using a 1.0 kb PCR fragment.
Advice
  • Recommendations: All pSpark® DNA cloning vectors are stable for at least 1 month at 4 °C and even at 20 – 25 °C for up to 2 days although storage temperatures above -20 °C are not recommended. In case of incident, such as a power failure, stored vectors should be tested with the supplied control insert before considering discarding it. However, please note that T4 DNA Ligase is extremely temperature-sensitive and storage temperatures above    -20 °C inactivates the enzyme.
Storage, Shipping & Guarantee
  • Shipped in: Gel Pack.
  • Storage: -20º C (NON Frost-Free Freezer).
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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