0.5M EDTA (pH 8.0)

Pouch format:
– One pouch containing precisely pre-weighed powder to prepare 500 mL of 0.5 M EDTA Buffer, pH 8.0.

Aqueous solution format:
– 100 mL of 0.5M EDTA Solution, Sterile, supplied as a ready-to-use liquid solution.

• Preparation of TE, TAE and TBE buffers.
• Protection of DNA and RNA from metal-dependent nuclease activity.
• Nucleic-acid purification, handling and storage workflows.
• Chelation of divalent metal ions such as Ca²⁺ and Mg²⁺.
• Molecular biology and biochemical buffer preparation.
• Cell-culture workflows requiring calcium chelation.

Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:

• Batch-to-batch consistency.
• Facilities audited regularly Quality audits.
• Lot specific CoAs and QA review previously to release a product.
• Material traceability, documented procedures and documented employee training.
• Equipment maintenance and monitoring records.

Request a Certificate of Analysis (CoA)

For BR0061 — Ready-to-use solution

• The product is supplied at its final working concentration and does not require dilution.
• Mix gently before use.
• Use appropriate clean or sterile handling techniques according to the intended application.

For BR0060 — Powder pouch

• Empty the complete contents of one pouch into a suitable flask or beaker.
• Add 500 mL of purified water and mix until completely dissolved.
• Verify that the powder has fully dissolved before use.
• Use an appropriate sterilization or filtration procedure when required by the intended application.
• Store the unopened pouch in a dry place at room temperature.

BR0061 — Ready-to-use solution

• Shipping: Ambient temperature.
• Storage: Room temperature.
• Keep the container tightly closed and prevent contamination during use.

BR0060 — Powder pouch

• Shipping: Ambient temperature.
• Storage: Store unopened in a dry place at room temperature.
• After preparation, store the resulting solution according to the internally validated conditions for 0.5 M EDTA Buffer.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

EDTA solution 0.5M, EDTA buffer pH 8.0, Chelating agent EDTA solution, Metal ion chelator EDTA, EDTA reagent solution

Also known as:

• Spanish: Solución de EDTA 0.5M pH 8.0, Agente quelante EDTA, Tampón EDTA
• French: Solution EDTA 0.5M pH 8.0, Agent chélateur EDTA, Tampon EDTA
• German: EDTA-Lösung 0,5M pH 8.0, Chelatbildner EDTA, EDTA-Puffer
• Italian: Soluzione EDTA 0.5M pH 8.0, Agente chelante EDTA, Tampone EDTA

Q: What formats are available?

A: The product is available as a 100 mL ready-to-use liquid solution, reference BR0061, and as a pre-weighed powder pouch that prepares 500 mL, reference BR0060.

Q: Is the pouch ready to use?

A: No. The pouch contains precisely pre-weighed powder and must be dissolved in purified water before use.

Q: Does the liquid solution require dilution?

A: No. BR0061 is supplied as a ready-to-use 0.5 M EDTA solution at pH 8.0.

Q: Are both formats sterile?

A: The liquid format is 0.22 µm filtered and aseptically filled. The sterility status of the powder pouch and of the solution prepared by the user depends on the applicable manufacturing and preparation controls.

Q: Can the product be used to prepare electrophoresis buffers?

A: Yes. EDTA is commonly used as a component of TE, TAE and TBE buffers.

Q: How should the pouch be prepared?

A: Empty the complete contents of one pouch into a suitable container, add 500 mL of purified water and mix until fully dissolved.