TBS Buffer (pH 7.4)

• Chemicals: Analytical grade.
• Composition: 0.050 M Tris-HCl, 0.15 M NaCL.
• pH: 7.4 ± 0.05 at 25 ºC.
• Sterilization: purified using a 0.22 µm filtration process [aseptically filled].
• Appearance: Clear, colorless solution
• Purity: DNase-, RNase-, and protease-free

Includes for 1L:

– 1,000 mL TBS Buffer solution in PET bottle.

Includes for 5L:

– 5 L TBS Buffer solution in 5L graduated square containers with moulded hand grip.

• Washing buffer in ELISA and Western blot assays.

• Antibody dilution buffer for immunoassays.

• Blocking solution to reduce background noise.

• Immunohistochemistry and hybridization protocols.

• General laboratory reagent for protein analysis.

• Each lot is tested for pH accuracy, conductivity, sterility, and absence of nucleases or proteases, ensuring maximum reproducibility.

• Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
  • • Batch-to-batch consistency.
    • Facilities audited regularly Quality audits.
    • Lot specific CoAs and QA review previously to release a product.
    • Material traceability, documented procedures and documented employee training.
    • Equipment maintenance and monitoring records.

  Request a Certificate of Analysis (CoA)

• Use directly without dilution.

• Keep container tightly closed after use.

• Mix gently before dispensing to maintain homogeneity.

• Avoid foaming during pipetting or pouring.

• Shipped at: Ambient Temperature.
• Storage: Room Temperature.

• Clua‐Ferré, L., De Chiara, F., Rodríguez‐Comas, J., Comelles, J., Martinez, E., Godeau, A. L., … & Ramón‐Azcón, J. (2022). Collagen‐Tannic Acid Spheroids for β‐Cell Encapsulation Fabricated Using a 3D Bioprinter. Advanced Materials Technologies, 2101696.
• Velasco-Mallorquí, F., Rodríguez-Comas, J., & Ramón-Azcón, J. (2021). Cellulose-based scaffolds enhance pseudoislets formation and functionality. Biofabrication, 13(3), 035044.
• Velasco-Mallorquı, Ferran, et al. “New volumetric CNT-doped gelatin–cellulose scaffolds for skeletal muscle tissue engineering.”
• Gómez-Domínguez, D., Epifano, C., de Miguel, F., Castaño, A. G., Vilaplana-Martí, B., Martín, A., … & Rodríguez-Milla, M. A. (2020). Consequences of Lmna Exon 4 Mutations in Myoblast Function.
• Machuca, M. A. O., Garibay, X. G. F., Castaño, A. G., De Chiara, F., Albors, A. H., Trias, J. B., & Azcon, J. R. (2019). Muscle-on-a-chip with on-site multiplexed biosensing system for in situ-monitoring of secreted IL-6 and TNF-α. Lab on a Chip.
• Hernández-Albors, A., Castaño, A. G., Fernández-Garibay, X., Ortega, M. A., Balaguer, J., & Ramón-Azcón, J. (2019). Microphysiological sensing platform for an in-situ detection of tissue-secreted cytokines. Biosensors and Bioelectronics: X, 100025.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the pH of TBS Buffer?
The buffer has a pH of 7.4 ± 0.1, optimized for immunoassay and protein analysis applications.

Q2: Is the buffer sterile?
Yes. It is sterile and ready-to-use, filtered through 0.2 µm membranes to ensure maximum purity.

Q3: Can it be used for antibody dilutions?
Yes. It provides excellent protein stability and reproducibility in antibody-based assays.