Tris-Glycine Buffer (pH 8.3) (10x)

High-quality 10× Tris-Glycine buffer (pH 8.3) for reliable protein electrophoresis performance.

Tris-Glycine Buffer (pH 8.3, 10×) from Canvax™ is a sterile, ready-to-dilute electrophoresis buffer designed for SDS-PAGE and native PAGE applications. It provides a stable ionic environment to maintain constant current, uniform migration, and optimal protein separation during electrophoresis and transfer procedures.

Manufactured in Canvax’s ISO 13485:2016 and GMP-certified facility, under ISO 9001:2016 quality standards, this buffer ensures outstanding purity, reproducibility, and batch-to-batch consistency, delivering sharp, well-defined protein bands and reliable results in both research and diagnostic workflows.

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Product available under ISO13485 / cGMP or with custom formulation, packaging or application. Learn more >

Price: 35.53 

SKU: BR0055 Categories: ,

Detailed information:

Advantages & Features
  • Ready-to-use concentrate: Supplied as a 10× stock solution for quick preparation of 1× working buffer.

  • High-quality manufacturing: Produced in an ISO 13485 and GMP certified plant, under an ISO 9001 standard for maximum reliability.

  • Optimized pH stability: Ensures consistent protein migration and reproducible results.

  • Exceptional purity: Free from DNase, RNase, and protease contamination.

  • Universal compatibility: Suitable for SDS-PAGE and native PAGE electrophoresis.

  • Excellent reproducibility: Guarantees consistent electrophoretic profiles and sharp protein bands.

Specifications
  • Chemicals: Analytical grade.
  • Composition: 0.025 M Tris, 0.192 M glycine.
  • pH: 8.3 ± 0.2 at 25 °C.
  • Sterilization: purified using a 0.22 µm filtration process [aseptically filled].
Includes

– 1,000 mL of Tris-Glycine Buffer Aqueous solution format

Download documentation
Datasheet
MSDS
Applications
  • Protein electrophoresis (SDS-PAGE and native PAGE).

  • Western blot transfer systems.

  • Protein purity and molecular-weight analysis.

  • Protein–protein interaction studies.

  • General electrophoretic procedures.

Tables & Figures

Quality Control
  • Each lot is tested for pH accuracy, conductivity, ionic strength, and enzymatic purity.
  • Manufactured in an ISO 13485:2016 and GMP-certified facility, located in Boecillo, Valladolid (Spain), under ISO9001:2016 procedures to ensure the highest quality standards. Our processes include:
      • Batch-to-batch consistency.
      • Facilities audited regularly Quality audits.
      • Lot specific CoAs and QA review previously to release a product.
      • Material traceability, documented procedures and documented employee training.
      • Equipment maintenance and monitoring records.

    Request a Certificate of Analysis (CoA)

Advice
  • Dilute 1 part 10× buffer with 9 parts deionized water.

  • Mix thoroughly before use.

  • Use freshly prepared 1× solution for optimal performance.

  • Store tightly closed to prevent contamination.

Storage, Shipping & Guarantee
  • Shipped at: Room temperature.
  • Storage: Room temperature.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review
FAQs

Q1: What is the recommended dilution ratio?
Dilute the 10× stock solution 1:9 with deionized water to prepare a 1× working buffer.

Q2: Can this buffer be used for Western blot transfer?
Yes. It is ideal for protein transfer when combined with methanol in standard protocols.

Q3: Does the buffer contain SDS?
No. It is supplied without SDS to allow flexible use in both native and SDS-PAGE.

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