UltraPure Water, DNASE/RNASE – Free

UltraPure DNase/RNase-Free water for molecular biology applications, ideal for PCR, qPCR, sequencing, cloning, and DNA/RNA purification.

The UltraPure Water DNase/RNase-Free is a highly purified, nuclease-free molecular biology grade water designed for sensitive DNA and RNA applications. This ultrapure water undergoes reverse osmosis, continuous deionization, UV treatment, distillation, and 0.2 µm filtration to eliminate contaminants and enzymes.

Each lot of this DNase/RNase-Free water is stringently tested to confirm the absence of DNase, RNase, and protease activity, ensuring reproducibility and reliability in PCR, qPCR, sequencing, cloning, and nucleic acid purification. With conductivity <2 µS/cm and pH between 5.4–7.0, it meets the highest quality standards for molecular biology workflows.

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WFI Quality Water also avaible. Learn more >

 

(6 customer reviews)

Price: 27.80 42.80 

SKU: E0322 Categories: ,

Detailed information:

Advantages & Features

  • Nuclease-free: DNase, RNase, and protease not detected.
  • Ultra pure: conductivity <2 µS/cm.
  • High quality: stringent QC for reproducibility.
  • Reliable: consistent lot-to-lot performance.
  • Convenient: ready-to-use for PCR, qPCR, cloning, and sequencing.

Specifications

  • CAS Number: 7732-18-5
  • Molecular Formula: H2O
  • Molecular Weight: 18.02
  • Filtration: filtered through a 0.3μ filter and tested for DNase-, RNase- & Protease activity.
  • Conductivity at 25º C < 2µS/cm
  • pH at 25º C 5.4 – 7.0
  • Purification process: includes continuous deionization, reverse osmosis, ultraviolet light treatment, distillation, and filtration.

 

Includes

Includes for 1 liter:
– 1,000mL of UltraPure Water, DNase-, RNase- & Protease-Free (Molecular Biology Grade)

Download documentation

Datasheet
MSDS

Applications

  • PCR and qPCR reactions.

  • DNA/RNA purification workflows.

  • Sequencing and library preparation.

  • Gene mapping and cloning.

  • Northern and Southern blotting.

Tables & Figures

Quality Control

Manufactured according to Strict quality control standards to ensure lot-to-lot consistency:

  • DNase: Not detected.
  • RNase: Not detected.
  • Protease: Not detected.
  • pH: pass.
  • Conductivity: Pass.

Advice

  • Use sterile technique to avoid contamination.

  • Do not autoclave (already sterile and nuclease-free).

Storage, Shipping & Guarantee

  • Shipment at Ambient Temperature.
  • Storage: Room Temperature.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

Synonym(s)

Nuclease-free water, DNase-free RNase-free water, Molecular biology grade water, PCR grade water, Ultrapure water for molecular biology

Also known as:

  • Spanish: Agua libre de nucleasas, Agua libre de DNasa y RNasa, Agua grado biología molecular
  • French: Eau sans nucléases, Eau sans DNase ni RNase, Eau qualité biologie moléculaire
  • German: Nukleasefreies Wasser, DNase- und RNase-freies Wasser, Wasser für Molekularbiologie
  • Italian: Acqua priva di nucleasi, Acqua senza DNasi e RNasi, Acqua per biologia molecolare

FAQs

Q: Is the water certified nuclease-free?
A: Yes, tested for DNase, RNase, and protease.

Q: Can it be used in PCR and qPCR?
A: Yes, it is validated for sensitive DNA and RNA reactions.

Q: What’s the difference vs. WFI water?
A: WFI is pharmaceutical-grade, while UltraPure DNase/RNase-Free is optimized for molecular biology.

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    6 reviews for UltraPure Water, DNASE/RNASE - Free

    1. Yuki Tanaka

      We use this DNase and RNase free water for sensitive molecular biology applications, including PCR and RNA-related work. It integrates easily into our workflows without requiring additional treatment steps. The specification is clearly indicated, which is important for our experimental reproducibility. It has been reliable for routine lab use.

    2. Nicolas Mertens

      Finding high-quality DNase-free water was simple on this website because of the detailed and clear descriptions provided. The delivery was very efficient, and I appreciated the ease of the whole buying experience from start to finish. This product helped me overcome challenges with enzymatic reactions that are highly sensitive to impurities. My experiments are now more reproducible, which has saved me a lot of time and resources in the long run. Overall, it is a top-tier product that I trust for all my molecular research.

    3. Bruno

      The purchase of this Ultrapure Water was straightforward, and I found the website very user-friendly for placing a quick order. Shipping was fast and the product was delivered in perfect condition, ready for immediate use in the lab. This water solved my problem with inconsistent buffer preparation, providing a clean and reliable base for my solutions. The quality is great, and it has definitely helped stabilize my experimental conditions. I am giving four stars only because I hope to see a wider variety of bottle sizes in the future.

    4. Lucas Lefebvre

      I bought this ultrapure water for sensitive reagent preparation, and the entire transaction was smooth and professional. The delivery was fast, and the packaging ensured the product remained pristine during transit to my facility. This water solved my issue with background noise in sensitive assays, proving it is of the highest quality and free of nucleases. It is a relief to find a product that performs exactly as advertised without any complications. I am very satisfied with both the product performance and the service.

    5. Thomas Janssens

      My experience purchasing this DNase/RNase-free water was excellent, from the intuitive checkout process to the prompt delivery. The product arrived well-sealed and exactly as described, which is vital for maintaining a sterile lab environment. It effectively solved the contamination issues I was facing in my DNA sequencing projects, ensuring high purity in every batch. The quality of this water has significantly improved the accuracy of my data. I highly recommend this product for anyone requiring high-grade ultrapure water for sensitive applications.

    6. Marc Dubois

      I ordered this Ultrapure Water for my RT-qPCR experiments, and I was very pleased with how clearly the product specifications were described on the website. The ordering process was quick and easy, and the package arrived in Brussels much faster than expected. This water is truly RNase-free, which solved my previous problems with RNA degradation during sample preparation. It has provided me with the consistent results I need for my molecular biology research. I will certainly continue to use this reliable source for my lab supplies.

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