FastPANGEA™ High Fidelity DNA Polymerase
FastPANGEA™ High Fidelity DNA Polymerase is a second-generation, proofreading enzyme that delivers extreme accuracy, speed, and robustness for all PCR applications.
With an error rate at least 50× lower than standard Taq DNA Polymerase, it amplifies long and complex templates (up to 15 kb) with blunt-end product generation — ideal for high-precision molecular cloning and sequencing workflows.
FastPANGEA™ High Fidelity DNA Polymerase sets a new benchmark for PCR accuracy, yield, and performance.
As a second-generation high-fidelity enzyme, it provides a unique balance of speed, robustness, and ultra-low error rate, making it suitable for both routine and demanding PCR applications.
This advanced enzyme possesses 5′→3′ DNA polymerase and 3′→5′ exonuclease (proofreading) activities, ensuring accurate nucleotide incorporation and minimal mutation rate.
It efficiently amplifies long DNA fragments (10–15 kb) and GC-rich or structurally difficult templates with extension times of only 15–30 seconds per kb.
The resulting PCR products are blunt-ended, enabling direct cloning into blunt-end or GC-cloning vectors.
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Price: 182.00 €
Detailed information:
Advantages & Features
- Second-generation enzyme: improved fidelity and robustness.
- Extreme accuracy: ≥ 50× lower error rate than Taq DNA Polymerase.
- Fast extension: 15–30 s / kb.
- High yield: superior product recovery with minimal enzyme amount.
- Robust performance: reliable results across long or difficult templates.
- Blunt-end PCR products: perfect for blunt-end or GC cloning.
- Versatile: ideal for routine PCR, long-range, or high-throughput applications.
Specifications
Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.
Includes
– 100 U FastPANGEA™ High Fidelity DNA Polymerase (2 U/μL)
– 1.5 mL Buffer UNI (2.5x)
– 100 μL DMSO
– 100 μL MgCl2 (25 mM)
Download documentation
Datasheet MSDSApplications
- PCR cloning and blunt-end ligation.
- Primer extension and mutagenesis.
- Long or difficult template amplification (10–15 kb).
- High-throughput and multiplex PCR.
- Sequencing library construction.
Tables & Figures
Quality Control
- Functionally tested in PCR.
- Undetected bacterial DNA (by PCR).
Advice
- Note: Highly recommended for cloning into pSpark® DNA cloning vectors.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- CHAABENE, R. B. Role of associated rhoptry proteins in Toxoplasma gondii invasion.
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Nyonda, M. A., Kloehn, J., Sosnowski, P., Krishnan, A., Lentini, G., Maco, B., … & Soldati-Favre, D. (2022). Ceramide biosynthesis is critical for establishment of the intracellular niche of Toxoplasma gondii. Cell reports, 40(7), 111224.
- Lentini, Gaëlle, et al. “Structural insights into an atypical secretory pathway kinase crucial for Toxoplasma gondii invasion.” Nature Communications 12.1 (2021): 1-17.
- Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
- García-Maceira, T., García-Maceira, F. I., González-Reyes, J. A., & Paz-Rojas, E. (2020). Highly enhanced ELISA sensitivity using acetylated chitosan surfaces.BMC Biotechnology, 20(1), 41.
- Otero Bilbao, A. (2014). Characterization of two metallocarboxypeptidases of M14D subfamily from bacterial and human origin.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
High fidelity DNA polymerase, Proofreading DNA polymerase, High accuracy PCR enzyme, DNA polymerase with proofreading activity, High fidelity PCR enzyme
Also known as:
- Spanish: Polimerasa de ADN de alta fidelidad, Polimerasa con proofreading, Enzima PCR de alta precisión
- French: ADN polymérase haute fidélité, Polymérase avec activité proofreading, Enzyme PCR haute précision
- German: Hochfidele DNA-Polymerase, Polymerase mit Proofreading, Hochpräzises PCR-Enzym
- Italian: DNA polimerasi ad alta fedeltà, Polimerasi con proofreading, Enzima PCR alta precisione
FAQs
1 review for FastPANGEA™ High Fidelity DNA Polymerase
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Lukas Schneider –
We incorporated this high-fidelity polymerase into our cloning workflow for amplifying several constructs above 3 kb. The enzyme is supplied with the corresponding buffer, which simplifies reaction setup and reduces variability between users. Cycling conditions were easy to adapt from our previous protocol, and amplification profiles were clean on agarose gels. It fits well in projects where sequence accuracy is critical before Sanger verification.