Horse-Power™ Green-Taq DNA Polymerase
Thermostable Taq polymerase with green dye for direct gel loading after PCR.
The Horse-Power™ Green Taq DNA Polymerase is a reliable and thermostable DNA polymerase engineered for high-yield PCR amplification across a wide range of DNA templates. Supplied with a green loading dye, it allows direct gel electrophoresis after PCR without additional sample preparation steps.
This enzyme combines robust performance, fidelity, and convenience, making it ideal for routine applications such as colony PCR, genotyping, cloning, and DNA fragment analysis. It provides excellent reproducibility and compatibility with standard PCR workflows.
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Price: 57.30 €
Detailed information:
Advantages & Features
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Direct-to-gel format: green dye allows direct loading on agarose gels.
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High yield: provides robust amplification of diverse templates.
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Reliable performance: ensures consistent results in routine PCR.
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Broad compatibility: suitable for plasmid, genomic, and cDNA templates.
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Convenient use: supplied in an optimized 10× reaction buffer.
Specifications
Assay conditions:
25 mM Tris-HCl pH 9.0 at 25 °C, 50 mM KCl, 2 mg MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Unit definition:
One unit is defined as the amount of enzyme required to catalyze the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Includes
• 100 µL Horse-Power Taq DNA polymerase (5U/µL).
• 2 x 1.25 mL 10X Green Buffer
Download documentation
Datasheet MSDSApplications
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Routine PCR amplification.
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Genotyping and colony PCR.
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Cloning and DNA fragment analysis.
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Educational and diagnostic research workflows.
Tables & Figures
Quality Control
- Each lot is tested for performance, activity, purity, and absence of endonuclease and exonuclease contamination.
Advice
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Mix gently before use.
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Optimize annealing temperature for best yield.
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Do not add additional dyes to the PCR reaction.
Storage, Shipping & Guarantee
• Shipped in: Gel pack.
• Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the advantage of the green format?
It includes a loading dye for direct agarose gel electrophoresis without extra steps.
Q2: Does it have proofreading activity?
No, Horse-Power™ Green Taq lacks 3’→5’ exonuclease activity, like standard Taq polymerase.
Q3: Can it be used for cloning applications?
Yes, PCR products have A-overhangs suitable for TA cloning.
