HotBegan™ Hot Start Green-Taq MasterMix
2× hot-start PCR MasterMix with green dye for specific amplification and direct gel loading.
The HotBegan™ Hot-Start Green Taq MasterMix is a 2× ready-to-use PCR reagent designed for high-specificity DNA amplification and direct gel electrophoresis. Featuring a chemically modified hot-start Taq polymerase, it prevents non-specific amplification and primer-dimer formation before the initial denaturation step, ensuring clean and accurate PCR results.
The integrated green loading dye enables direct sample loading on agarose gels, eliminating additional preparation steps. This mix delivers robust performance, excellent yield, and reproducibility across a wide range of DNA templates, including genomic, plasmid, and cDNA targets.
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Price: 105.84 €
Detailed information:
Advantages & Features
- Optimized for TA Cloning and GC Cloning.
- Accurate & Fast visual Tracking of DNA Migration: thanks to its composition that includes Green Dye Buffer.
- Powerful: amplification of targets up to 5 kb.
- Hot-start activation: prevents non-specific amplification and primer-dimers.
- Direct-to-gel format: green dye allows immediate agarose gel loading.
- High specificity: ensures clean, reproducible amplification results.
- Time-saving: reduces handling steps with a 2× ready-to-use mix.
- Broad compatibility: ideal for genomic, plasmid, and cDNA templates.
Specifications
- Assay Conditions: 25mM Tris-HCl pH9.0 at 25 °C, 50mM KCl, 2mM MgCl2, 0.1mg/mL gelatine, 200 μM of dATP, dGTP, dTTP, 100μM[α32-P]dCTP (0.05 μCi/nmol) and 12.5 μg activated salmon sperm DNA.
- Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes
– 5 x 1.25 mL HotBegan™ Hot Start Green-Taq MasterMix (2x)*, ready-to-use
– 1.5 mL 50mM MgCl2 Solution**
* Includes HotBegan™ Hot Start Taq DNA Polymerase, Green Dye buffer (2x), 0.4 mM of each dNTP, 4 mM Mg2+ and 24% Glycerol.
**Separate tube 50 mM MgCl2 solution is provided for further optimisation. In some cases, we recommend to optimize Mg2 + concentration.
Download documentation
Datasheet MSDSApplications
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High-specificity PCR amplification.
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Colony PCR and genotyping.
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DNA fragment cloning and sequencing.
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Routine molecular biology applications.
Tables & Figures
Quality Control
- Each batch is tested for amplification efficiency, enzyme activity, and absence of endonucleases or exonucleases.
Advice
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Mix gently before use; avoid vigorous shaking.
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For best performance, optimize annealing temperature.
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Store in aliquots to prevent repeated freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the main advantage of the hot-start feature?
It prevents non-specific amplification by activating the enzyme only after heating.
Q2: Does the green dye affect PCR performance?
No, it does not interfere with amplification and allows direct agarose gel loading.
Q3: Is this mix compatible with different DNA templates?
Yes, it works efficiently with genomic, plasmid, and cDNA templates.
