HotBegan™ Hot Start Taq-DNA Polymerase, MasterMix
High-specificity 2× PCR MasterMix with hot-start Taq polymerase for clean and reproducible amplification.
HotBegan™ Hot-Start Taq DNA Polymerase MasterMix provides a ready-to-use 2× PCR formulation containing chemically modified Taq DNA Polymerase, optimized buffer, MgCl₂, and dNTPs. The hot-start mechanism prevents premature polymerase activity at lower temperatures, ensuring superior specificity and cleaner amplification.
This formulation minimizes pipetting steps and variability, delivering consistent results in diagnostic PCR, low-copy target amplification, and multiplex assays. It is ideal for users seeking reliable, high-quality results with reduced background noise and maximum convenience.
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Price: 66.87 €
Detailed information:
Advantages & Features
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Hot-start activation: Prevents non-specific amplification and primer–dimer formation.
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Ready-to-use 2× mix: Simplifies reaction setup for routine or diagnostic PCR.
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High specificity: Produces clean, reproducible amplification results.
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Reduced contamination risk: Minimizes handling steps and pipetting errors.
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Consistent performance: Delivers batch-to-batch reproducibility.
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Compatible with complex templates: Suitable for genomic and cDNA targets.
Specifications
Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.
Includes
– 2 x 1.25 mL HotBegan™ Hot Start Taq DNA Polymerase MasterMix, which includes HotBegan™ Hot Start Taq DNA Polymerase (0.1 U/μL), 2x PCR Buffer, 0.4 mM of each dNTP, 4 mM Mg2+, 4% Glycerol.
– 2 x 1.25 mL Nuclease-free water.
Download documentation
Datasheet MSDSApplications
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High-specificity and diagnostic PCR.
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Low-copy DNA target amplification.
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Multiplex PCR and complex templates.
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Routine PCR workflows requiring accuracy.
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Educational and research applications.
Tables & Figures
Quality Control
- Each batch is tested for specificity, activity, and absence of DNase/RNase contamination, ensuring consistent PCR yields and reproducible results.
Advice
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Include an initial activation step at 95 °C for 2–10 min.
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Mix gently before use; avoid vigorous shaking.
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Store aliquots to prevent repeated freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- Albuquerque, C. A. D. (2019). Detection and characterization of Dichelobacter nodosus from sheep with different clinical manifestations of Ovine Footrot (Doctoral dissertation).
- Osset Iborra, H. (2019). Seguimiento de las plagas de Tomicus destruens y Orthotomicus erosus y detección de Botryosphaeriaceae, hongos patógenos asociados a estos insectos, en los pinares de Pinus halepensis de La Pedrera y la Devesa del Saler (Valencia) (Doctoral dissertation).
- Negreiros, A. M., Júnior, R. S., Rodrigues, A. P., León, M., & Armengol, J. Prevalent weeds collected from cucurbit fields in Northeastern Brazil reveal new species diversity in the genus Monosporascus. Annals of Applied Biology.
- Martín-Pinto, P. Fungal communities from forest systems in Ethiopia.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession under Eucalyptus grandis plantations in Ethiopia. Forest Ecology and Management, 405, 179-187.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.
- Huyghe, J. (2017). Selection of reference genes for gene expression studies in Lactic acid bacteria.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal community succession and sporocarp production following fire occurrence in Dry Afromontane forests of Ethiopia. Forest Ecology and Management, 398, 37-47.
- Criado-García, J., Fuentes, F., Cruz-Teno, C., García-Rios, A., Jiménez-Morales, A., Delgado-Lista, J., … & Pérez-Jiménez, F. (2011). R353Q polymorphism in the factor VII gene and cardiovascular risk in Heterozygous Familial Hypercholesterolemia: a case-control study. Lipids in health and disease, 10(1), 50.
- Català, S., Pérez-Sierra, A., & Abad-Campos, P. (2015). The use of genus-specific amplicon pyrosequencing to assess Phytophthora species diversity using eDNA from soil and water in northern Spain. PloS one, 10(3), e0119311.
- Olmo, D., Armengol, J., León, M., & Gramaje, D. (2016). Characterization and Pathogenicity of Botryosphaeriaceae Species Isolated from Almond Trees on the Island of Mallorca (Spain). Plant Disease, 100(12), 2483-2491.
- Dejene, T., Oria-de-Rueda, J. A., & Martín-Pinto, P. (2017). Fungal diversity and succession following stand development in Pinus patula Schiede ex Schltdl. & Cham. plantations in Ethiopia. Forest Ecology and Management, 395, 9-18.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What advantage does the hot-start mechanism offer?
It prevents enzyme activity at room temperature, avoiding primer–dimer and nonspecific amplification.
Q2: Can it be used for multiplex PCR?
Yes. It provides excellent specificity and reproducibility in multiplex assays.
Q3: Is it compatible with dye-based detection?
Yes. It can be used in combination with loading dye or visualization reagents.
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