Horse-Power™ Red DNA Polymerase MasterMix
Ready-to-use 2× PCR MasterMix with red loading dye for fast, reproducible DNA amplification
Horse-Power™ Red DNA Polymerase MasterMix offers a ready-to-use 2× PCR solution optimized for consistent, high-quality DNA amplification. It contains Taq DNA Polymerase, reaction buffer, MgCl₂, dNTPs, and red loading dyes that allow direct loading of PCR products onto agarose gels without additional steps.
This formulation minimizes setup time, reduces pipetting errors, and ensures excellent reproducibility across assays. It is ideal for routine PCR, genotyping, and colony screening, delivering reliable amplification and vivid visual tracking during electrophoresis.
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Price: 44.95 €
Detailed information:
Advantages & Features
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Ready-to-use format: Simply add template DNA and primers.
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Integrated red loading dye: Enables direct agarose gel loading after PCR.
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Time-saving workflow: Reduces pipetting and minimizes setup time.
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Reliable performance: Consistent amplification efficiency across samples.
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Reduced variability: Pre-optimized formulation ensures reproducibility.
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Excellent visualization: Dual dye system provides clear sample tracking.
Specifications
Concentration:
2.5x (Buffer Red 2.5X, TruePure™ dNTPs 0.5 mM each, HorsePower™ Taq DNA Polymerase 0.25 U/μL, Glycerol 30%).
Assay conditions:
25 mM Tris-HCl pH9.0 at 25 °C, 50 mM KCl, 2 mM MgCl2, 0.1 mg/mL gelatine, 200 µM dATP, dGTP, dTTP, 100 µM [α32-P] dCTP (0.05 µCi/nmol) and 12.5 µg activated salmon sperm DNA.
Includes
– 100 rxn Horse-Power™ Red DNA Polymerase Master Mix (2.5x)
*Includes all necessary reagents except template and primer DNA.
Download documentation
Datasheet MSDSApplications
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Routine PCR and DNA amplification.
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Genotyping and colony PCR.
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DNA fragment analysis and cloning.
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Educational or diagnostic research.
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Direct agarose gel loading without extra buffer.
Tables & Figures
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Quality Control
- Each batch is tested for amplification efficiency, specificity, yield reproducibility, and absence of DNase/RNase contamination, ensuring consistent PCR performance.
Advice
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Mix gently before use to avoid bubbles.
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Use 10–100 ng DNA template per reaction.
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Store aliquots to prevent repeated freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- Kaddouri, A. C., ElOirdi, S., Lakhlifi, T., Miyah, Y., Benjelloun, M., Zakya, M. H., & Belhaj, A. (2025). Isolation and characterization of three Lactiplantibacillus plantarum strains with high activity and broad antifungal spectrum.NRFHH 2025;5(2):196-208
- Stefanova, P., Georgieva, A., Brazkova, M., Baldzhieva, R., Goranov, B., Blazheva, D., … & Angelova, G. (2025). Molecular Identification, Mycelial Growth Kinetics, and Antimicrobial Potential of Newly Isolated Medicinal Mushroom Fomitopsis pinicola from Bulgaria. Journal of Fungi, 11(10), 727.
- Angelova, G., Stefanova, P., Brazkova, M., & Krastanov, A. (2023). Molecular and morphological characterization of Xylaria karsticola (Ascomycota) isolated from the fruiting body of Macrolepiota procera (Basidiomycota) from Bulgaria. Plos one, 18(6), e0287679.
- Petrova, S., & Petkova, M. (2023). Plant Traits of Tilia tomentosa Moench, Fraxinus excelsior L., and Pinus nigra JF Arnold as a Proxy of Urbanization. Forests, 14(4), 800.
- Petkova, M., Gotcheva, V., Dimova, M., Bartkiene, E., Rocha, J. M., & Angelov, A. (2022). Screening of Lactiplantibacillus plantarum Strains from Sourdoughs for Biosuppression of Pseudomonas syringae pv. syringae and Botrytis cinerea in Table Grapes. Microorganisms, 10(11), 2094.
- Petkova, M., Spasova-Apostolova, V., Masheva, V., Atanasova, D., & Tahsin, N. (2021). Endophytic colonization of Solanaceае family plants by fungal entomopathogen Beauveria bassiana strain 339 to control Colorado potato beetle (Leptinotarsa decemlineata Say). Bulgarian Journal of Agricultural Science, 27, 1.
- Petkova, Mariana, et al. “Isolation and Characterization of Lactic Acid Bacteria and Yeasts from Typical Bulgarian Sourdoughs.” Microorganisms 9.7 (2021): 1346.
- Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.
- Ruiz Carrascoso, G. (2016). Características microbiológicas y clínico-epidemiológicas de enterobacterias productoras de carbapenemasa oxa-48 en el contexto de un brote hospitalario (Doctoral dissertation, Universidad Complutense de Madrid).
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the purpose of the red dye in this mix?
The red tracking dye allows direct loading of PCR products onto agarose gels without adding loading buffer.
Q2: Is it suitable for colony PCR?
Yes. It delivers consistent results for crude lysates and bacterial colonies.
Q3: What fragment length can it amplify?
Up to 5 kb, depending on template complexity and reaction optimization.
