HotBegan™ Hot Start Red DNA Polymerase MasterMix
2× hot-start PCR MasterMix with red dye for specific amplification and easy visualization.
The HotBegan™ Hot-Start Red Polymerase MasterMix is a 2× ready-to-use PCR reagent that delivers exceptional specificity and convenience for routine and demanding PCR applications. Its hot-start Taq polymerase remains inactive at low temperatures, preventing non-specific amplification and primer-dimer formation before the first denaturation step.
The built-in red tracking dye simplifies sample handling and allows direct loading onto agarose gels, eliminating additional loading buffer preparation. This MasterMix provides high yield, strong reliability, and consistent results, even with complex DNA templates.
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Price: 105.84 €
Detailed information:
Advantages & Features
- Accurate & Fast visual Tracking of DNA Migration: thanks to its composition that includes Red Dye Buffer.
- Powerful: amplification of targets up to 5 kb.
- Hot-start activation: eliminates non-specific amplification and primer-dimers.
- Red tracking dye: enables visual pipetting and direct gel loading.
- High specificity: ideal for complex or low-copy DNA targets.
- Ready-to-use: reduces setup time and experimental variability.
- Broad compatibility: works with genomic, plasmid, and cDNA templates.
Specifications
- Assay Conditions: 25mM Tris-HCl pH9.0 at 25°C, 50mM KCl, 2mM MgCl2, 0.1mg/mL gelatine, 200 µM of dATP, dGTP, dTTP, 100µM[α32-P]dCTP (0.05µCi/nmol) and 12.5 µg activated salmon sperm DNA.
- Unit Definition: One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74 °C.
Includes
Includes for 250 rxn:
– 5 x 1.25 mL HotBegan™ Hot Start Red DNA Polymerase MasterMix (2x)*
– 1.5 mL MgCl2 Solution (50mM)**
* Includes HotBegan™ Hot Start Taq DNA Polymerase, Red Dye buffer (2x), 0.4 mM of each dNTP, 5 mM Mg2+ and 5% Glycerol.
**Separate tube 50 mM MgCl2 solution is provided for further optimisation. In some cases, we recommend to optimize Mg2 + concentration.
Download documentation
Datasheet MSDSApplications
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High-specificity PCR amplification.
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Genotyping and colony PCR.
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DNA cloning and sequencing.
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Diagnostic and research PCR workflows.
Tables & Figures
Quality Control
- Each batch is tested for enzyme activity, performance, amplification efficiency, and absence of endonuclease or exonuclease contamination.
Advice
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Mix thoroughly before use; avoid bubble formation.
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Use standard cycling parameters for Taq-based PCR.
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Store in aliquots to prevent repeated freeze–thaw cycles.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
- Kaddouri, A. C., ElOirdi, S., Lakhlifi, T., Miyah, Y., Benjelloun, M., Zakya, M. H., & Belhaj, A. (2025). Isolation and characterization of three Lactiplantibacillus plantarum strains with high activity and broad antifungal spectrum.NRFHH 2025;5(2):196-208
- León, Maela, et al. “Identification and Characterization of Diaporthe spp. Associated with Twig Cankers and Shoot Blight of Almonds in Spain.“ Agronomy 10.8 (2020): 1062.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q1: What is the advantage of the red dye?
It allows visual pipetting and direct gel loading without extra buffer.
Q2: Does the red dye interfere with PCR or downstream applications?
No, it does not affect amplification or subsequent gel analysis.
Q3: Is this MasterMix suitable for complex DNA templates?
Yes, the hot-start mechanism ensures high specificity in difficult samples.
