FastPANGEA™ Long PCR DNA Polymerase, MasterMix
High-fidelity polymerase mix for robust amplification of long DNA fragments up to 15 kb.
The FastPANGEA™ Long PCR DNA Polymerase Master Mix combines a robust Taq DNA Polymerase with a proofreading enzyme possessing 3’→5’ exonuclease activity, enabling high-fidelity amplification of DNA fragments up to 15 kb.
This unique blend increases fidelity 6.5-fold compared to Taq alone, while ensuring strong amplification of long and difficult templates. The mix generates blunt-end PCR products and 3’-adenine overhangs, making them directly compatible with T-vectors for cloning.
With a fast and accurate protocol, this ready-to-use 2x Master Mix delivers reproducible results across genomic, cDNA, and complex templates.
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Price: 44.95 €
Detailed information:
Advantages & Features
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High fidelity: 6.5× greater accuracy than Taq alone.
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Robust amplification: fragments up to 15 kb.
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High yield & specificity.
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Versatile: works with long or GC-rich templates.
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Convenient: blunt and A-overhang products for easy cloning.
Specifications
Unit definition:
One unit is defined as the amount of enzyme that incorporates 10 nmoles of dNTP’s into acid-insoluble form in 30 minutes at 75 °C under assay conditions: 25 mM TAPS-HCl, pH 9.0 (at 25 °C), 100 mM KCl, 1.5 mM MgCl2, 1 mM Beta-mercaptoethanol, 200 μM each dNTP and 10 μg activated calf thymus DNA in 50 μl.
Includes
– 200 rxn FastPANGEA™-Long PCR Master Mix (2x)
– 50 μL DMSO (100%)
– 100 μL MgCl2 (25 mM)
*Separate tubes of DMSO and 25 mM MgCl2 solutions are provided for further optimisation.
Download documentation
Datasheet MSDSApplications
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Long-range PCR (5–15 kb genomic DNA).
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Cloning of long amplicons.
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Primer extension.
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High-throughput PCR.
Tables & Figures
Quality Control
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Functionally tested in long PCR with 5–15 kb genomic templates.
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Verified free of bacterial DNA by qPCR.
Advice
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Use DMSO for GC-rich templates.
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Optimize MgCl₂ concentration for long amplicons.
Storage, Shipping & Guarantee
- Shipped in: Gel pack.
- Storage: -20 °C.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
Q: What is the maximum fragment size?
A: Up to 15 kb with genomic DNA.
Q: Can it be used for cloning?
A: Yes, products include blunt ends and A-overhangs for T-vectors.
Q: Does it include optimization reagents?
A: Yes, separate tubes of DMSO and MgCl₂ are included.
