Proteinase K lyophilized powder 30 mg – Canvax

Proteinase K Powder

Name: Proteinase K Powder
SKU: EZ0011
Availability: InStock

High-quality Proteinase K lyophilized powder ≥30 units/mg, nuclease-free, ideal for DNA/RNA extraction and protein digestion

The Proteinase K Lyophilized Powder ≥30 units/mg is a highly active, broad-spectrum serine protease purified from Tritirachium album. This enzyme exhibits wide cleavage specificity, hydrolyzing peptide bonds adjacent to the carboxylic group of aliphatic and aromatic amino acids.

Thanks to its stability and activity across a wide pH range (4–12.5), and resistance to denaturants such as SDS and EDTA, this Proteinase K lyophilized powder is ideal for digesting proteins during DNA and RNA extraction workflows. Its high purity, free from RNases, DNases, and exonucleases, ensures reliable results in molecular biology applications.

The lyophilized format allows convenient storage, shipping at ambient temperature, and reconstitution when needed.

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Price: 47.14 € – 318.71 €

size

30 mg

100 mg

500 mg

SKU: EZ0011 Categories: End Point PCR, Related Enzymes & Products

Detailed information:

Advantages & Features

High activity: ≥30 units/mg protein.
Stable & versatile: active across pH 4–12.5, resistant to SDS and EDTA.
High purity: nuclease-free (DNase, RNase, exonuclease not detected).
Convenient format: lyophilized powder, easy to store and reconstitute.
Chromatographically purified: ensures reproducibility and reliability.

Specifications

Unit Definition: Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
Host: Komagataella phaffii (Pichia pastori).
Appearance: White lyophilized powder.
Source: Tritirachium album.
Activity: ≥30 units/mg protein.
Molecular weight: 28.9 kDa.
pH range 4.0 – 12.5.

Includes

Includes for 30 mg:
– 30 mg Proteinase K (Lyophilized powder, =30 units/mg)

Download documentation

Datasheet

MSDS

Applications

Isolation of genomic DNA and RNA from cells and tissues.
Removal of nucleases during DNA/RNA purification.
Protein digestion for molecular biology workflows.
Enzyme localization studies.

Tables & Figures

Quality Control

Enzyme activity is assayed by digesting hemoglobin at a concentration of 16.7 mg/mL in a solution of 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl₂.
Free of detectable RNase, DNase and exonuclease activities.

Advice

Reconstitute in sterile water or buffer before use.
Avoid repeated freeze-thaw cycles.
Use RNase-free consumables for best results.

Storage, Shipping & Guarantee

Shipment at Ambient Temperature.
Storage: 4 °C or -20 °C.

Citations

Riascos-España, A. F., Cuastumal, B. T. A., Zambrano, M. C. I., Arteaga, J. Z. C., & Velasquez-Vasconez, P. A. (2025). Optimized Protocol for DNA Extraction in Three Theobroma Species. Bio-protocol, 15(9), e5297.
Chekli, Y., Peron-Cane, C., Dell’Arciprete, D., Allemand, J. F., Li, C., Ghigo, J. M., … & Beloin, C. (2020). Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Scientific reports, 10(1), 1-13.
Merinas-Amo, R. (2021). Papel de aditivos alimentarios en la modulación de procesos degenerativos: estudios in vivo e in vitro.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.