Proteinase K Powder

• Unit Definition: Unit definition One unit is the amount of enzyme which releases at 37 °C in 1 min as many folin-positive amino acids and peptides from hemoglobin as 1 μmol of tyrosine.
• Host: Komagataella phaffii (Pichia pastori).
• Appearance: White lyophilized powder.
• Source: Tritirachium album.
• Activity: ≥30 units/mg protein.
• Molecular weight: 28.9 kDa.
• pH range 4.0 – 12.5.

Includes for 30 mg:
– 30 mg Proteinase K (Lyophilized powder, =30 units/mg)

• Isolation of genomic DNA and RNA from cells and tissues.

• Removal of nucleases during DNA/RNA purification.

• Protein digestion for molecular biology workflows.

• Enzyme localization studies.

• Enzyme activity is assayed by digesting hemoglobin at a concentration of 16.7 mg/mL in a solution of 0.08 M potassium phosphate (pH 7.5), 5 M urea, 4 mM NaCl, 3 mM CaCl₂.
• Free of detectable RNase, DNase and exonuclease activities.

• Reconstitute in sterile water or buffer before use.

• Avoid repeated freeze-thaw cycles.

• Use RNase-free consumables for best results.

• Shipment at Ambient Temperature.
• Storage: 4 °C or -20 °C.

• Riascos-España, A. F., Cuastumal, B. T. A., Zambrano, M. C. I., Arteaga, J. Z. C., & Velasquez-Vasconez, P. A. (2025). Optimized Protocol for DNA Extraction in Three Theobroma Species. Bio-protocol, 15(9), e5297.
• Chekli, Y., Peron-Cane, C., Dell’Arciprete, D., Allemand, J. F., Li, C., Ghigo, J. M., … & Beloin, C. (2020). Visualizing the dynamics of exported bacterial proteins with the chemogenetic fluorescent reporter FAST. Scientific reports, 10(1), 1-13.
• Merinas-Amo, R. (2021). Papel de aditivos alimentarios en la modulación de procesos degenerativos: estudios in vivo e in vitro.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Proteinase K powder, Protein digestion enzyme powder, Protease K lyophilized, Broad-spectrum protease powder, Protein degradation enzyme

Also known as:

• Spanish: Proteinasa K en polvo, Enzima digestión de proteínas, Proteasa K liofilizada
• French: Protéinase K en poudre, Enzyme digestion des protéines, Protéase K lyophilisée
• German: Proteinase K Pulver, Proteinabbauenzym, Lyophilisierte Protease K
• Italian: Proteinasi K in polvere, Enzima digestione proteine, Proteasi K liofilizzata

Q: How should Proteinase K be reconstituted?
A: Reconstitute in nuclease-free water or buffer just before use.

Q: Is it resistant to SDS and EDTA?
A: Yes, it remains active in the presence of detergents and chelating agents.

Q: Can it be used for both DNA and RNA extraction?
A: Yes, it efficiently digests proteins and removes nucleases for both workflows.