Poly A Polymerase
High-purity Poly(A) Polymerase (E. coli) for efficient ATP-dependent RNA polyadenylation, optimized for in vitro transcription and RNA research.
Poly(A) Polymerase (E. coli) adds polyadenylate tails to RNA molecules using ATP as a substrate. It is widely used for RNA stabilization, tailing, and library preparation in RNA-related studies.
This enzyme provides consistent activity and reliable results in in vitro transcription and RNA processing workflows.
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Detailed information:
Advantages & Features
- High-Purity: ≥ 95% determined by SDS.
- Recombinant production in E. coli, ensuring purity and consistency.
- Compatible with IVT workflows for mRNA vaccine and therapeutic development.
- Extensively quality controlled for reproducibility and reliability.
- High catalytic efficiency for fast and reliable poly(A) tailing.
- Flexible applications, from basic RNA research to industrial bioprocesses.
Specifications
Protein information
Predicted MW: 54 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
Buffer Storage: 1X Reaction Buffer: 20 mM Tris-HCl, 300 mM NaCl, 1 mM EDTA, 1 mM DTT, 0.1 % Triton®X-100, 50% Glycerol, (pH 7.5 at 25°C).
Biological activity: One unit is defined as the amount of enzyme that will incorporate 1 nmol of AMP into RNA in a 20 µl volume in 10 minutes at 37°C.
Gene Information
Synonyms: PAP I
Official Symbol: pcnB
Specie: Escherichia coli
GenBank accession number: NC_000913 (Range 157729..159126)
NCBI Protein RefSeq: NP_414685.4
Protein Family: polyA polymerase
Includes
– 100 U Poly(A) Polymerase, from E. coli (5 U/µL)
– Adenosine-5′-Triphosphate (ATP)
– Poly(A) Polymerase Reaction Buffer
Download documentation
Datasheet MSDSApplications
- In vitro transcription workflows.
- Labeling of RNA with ATP or cordycepin.
- Polyadenylation of synthetic RNA for stability & translation enhancement.
- Enhances translation of RNA transferred into eukaryotic cells.
- mRNA-based vaccine and therapeutic development.
- Functional RNA studies and transcriptome analysis.
Tables & Figures
Quality Control
Advice
For optimal results, always use RNase-free reagents and handle RNA samples with proper aseptic techniques. Avoid exposure to constant temperature changes.
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer).
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
To be informed about our Safety Staments for GMP Products, please contact us.
Customers Review
FAQs
Q1: Can Poly(A) Polymerase be used in IVT workflows for mRNA vaccines?
A: Yes, it is fully compatible with in vitro transcription and polyadenylation of synthetic mRNA.
Q2: Does the enzyme require specific cofactors?
A: The reaction requires ATP as substrate and Mg²⁺ as cofactor.
