T7 RNA Polymerase
High Quality & Proven Performance in RNA In Vitro Transcription Reactions
T7 RNA Polymerase is a ~99 kDa, DNA-dependent RNA polymerase engineered for high-yield, ultra-pure in-vitro transcription (IVT). Manufactured under ISO 13485 and cGMP principles, each lot is certified RNase-free, endotoxin-controlled and supported by a detailed Certificate of Analysis (CoA). The enzyme shows strict specificity for the T7 promoter, enabling efficient production of mRNA, sgRNA and RNA probes at laboratory or industrial scale. Have questions? Contact us
Price: 26.60 €
Detailed information:
Advantages & Features
- Isolated form a recombinant source (E. coli).
- Extensivelly quality controlled: ≥ 95% determined by SDS, Endo/exonucleases, non-specific DNAse and RNAses free, Promoter Specificity, etc.
- Concentration 50 U / µL for scalable reactions
- Minimal dsRNA & RNase contamination
- Compatible with capped, modified and pseudouridine NTPs
- Free of animal-derived components
Specifications
Protein information
Predicted MW: ~99 kDa
Purity: ≥ 95% as determined by SDS-polyacrylamide gels with Coomassie® blue staining.
Buffer: 10X Reaction Buffer: 40 mM Tris-HCl, 6 mM MgCl2, 1 mM DTT, 2 mM spermidine (pH 7.9 @ 25°C).
Biological activity: One unit is defined as the amount of enzyme required to generate 1 nmol RNA in 1 hour at 37°C determined by a fluorimetric method.
Gene Information
Synonyms: T7RNAP
Specie: Escherichia phage T7
GenBank accession number: NC_001604.1
NCBI Protein RefSeq: NP_041960.1
Protein Family: Family of single-subunit RNAPs that includes the phage RNAPs (T3,K11, SP6, N4, and others) as well as the mitochondrial RNAPs.
Includes
– 5,000 U T7 RNA Polymerase (50 U/µL)
– T7 RNA Polymerase Reaction Buffer (200 µL)
Download documentation
Datasheet MSDS BrochureApplications
- mRNA synthesis
- RNA standard template for RT-PCR
- RNA probes for hybridization.
- Studies of RNA secondary structure and RNA-protein interactions, RNA splicing.
- CRISPR sgRNA and gRNA synthesis
- In-vitro translation, microinjection & structural studies
Tables & Figures
Quality Control
Quality Tests includes:
- Appearance: Clear, colorless solution
- pH: 8 ± 0,3
- Purity & Identity by SDS-PAGE: ≥ 95 % and 97 ± 3 kDa
- Activity: 50 ± 10 U µL⁻¹
- Endonuclease Activity: No detectable
- Exonuclease Activity: No detectable
- RNAse Activity: No detectable
- Unspecific DNase Activity: No detectable
- Residual genomic DNA: < 350 pg/ml
- Endotoxins: ≤ 0,25 EU/1000U
- Bioburden: ≤ 10 CFU/10000U
Advice
- Use freshly linearised DNA templates free of RNase contamination.
- Maintain 1 mM DTT and 2–3 mM Mg²⁺ for optimal activity.
- For very long transcripts (>5 kb) extend incubation to 4 h at 37 °C.
- For extra yield see our Inorganic Pyrophosphatase (ref. EZ0035; see related products)
Storage, Shipping & Guarantee
- Shipped in: Gel Pack.
- Storage: -20 ºC (NON Frost-Free Freezer) in provided buffer.
- Avoid freeze-thaw cycles.
- Canvax guarantees enzyme activity for >24 months from manufacturing when stored as recommended.
Citations
Safety Statements
This product is developed, designed and sold exclusively for Research purposes use only (RUO). The product was not suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
FAQs
- Can I run primers-only PCR templates?
Yes; ensure the template contains a T7 promoter upstream of the target. - Is the enzyme compatible with CleanCap® analogs?
Fully compatible; follow supplier-recommended cap analog:NTP ratios. - How do I increase RNA yield further?
Consider adding Inorganic Pyrophosphatase (EZ0035) to remove inhibitory pyrophosphate.
