PRImeDETECT™ STEC Detection Kit

• Amplification limit:  5 DNA copies per reaction.
• Quantification Dynamic range: 4 logs.
• Simultaneously detect and differentiate the major STEC-associated virulence marker genes: stx1, stx2 and eae in the same PCR amplification reaction.
• Inclusivity: specifically reacts with all strains of E. coli with the indicated target genes (stx1, stx2 and eae).
• Compliance: Manufactured according to ISO/TS 13136:2012.
• Exclusivity: No cross-reactions with other strains of Non- Shiga toxin-producing E. coli (STEC).
• Detection: probe labelled with fluorescent dyes–  FAM-BHQ1a (sxt1), HEX-BHQ1(stx2), ROX-BHQ2 (eae) and CY5-BHQ1(IAC).
• Thermal cycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.

– 1 x Multiplex PCR MasterMix
– 1 x DNAready Lysis Buffer
– 1 x PCR Positive Control
– 1 x PCR Negative Control

• Detection of Shiga toxin-producing E. coli (STEC).

• Food safety testing for pathogenic E. coli.

• Environmental monitoring of STEC contamination.

• Rapid screening in microbiological workflows.

• Manufactured according to ISO/TS 13136:2012.
• Strict quality controls in every manufacturing step to guarantee the highest quality and reproducibility.

• Always run positive and negative controls with each assay.

• Use DNAready Lysis Buffer for efficient DNA extraction.

• Prevent contamination by separating PCR setup and sample handling areas.

• Shipment at Ambient Temperature.
• Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What genes does the PRImeDETECT™ STEC Detection Kit target?
It detects virulence genes stx1, stx2, and eae.

Q2: Does the kit include an internal control?
Yes, it contains an Internal Amplification Control (IAC) to monitor PCR inhibition.

Q3: Can the kit detect multiple genes in one reaction?
Yes, it performs multiplex PCR for simultaneous detection of all three targets.