qMAXSen™ Green qPCR MasterMix (Low ROX™)
High-sensitivity 2× qPCR master mix with Low ROX reference dye for precise and reproducible quantification.
qMAXSen™ Green qPCR MasterMix (Low ROX) from Canvax™ is a ready-to-use, 2× concentrated master mix optimized for high-performance quantitative PCR (qPCR) using intercalating dye detection. This advanced formulation provides exceptional sensitivity, reproducibility, and specificity, ensuring reliable quantification across a broad dynamic range.
The Low ROX concentration makes this mix fully compatible with qPCR platforms that require minimal passive reference dye, including Agilent, Stratagene, and certain Applied Biosystems instruments. The mix contains an optimized hot-start DNA polymerase, MgCl₂, dNTPs, and buffer system for robust, precise, and efficient amplification of low-copy templates.
Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®.
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Price: 218.70 €
Detailed information:
Advantages & Features
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High sensitivity: Detects low-copy targets with strong fluorescence signal and minimal background.
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Low ROX compatibility: Ideal for qPCR instruments requiring minimal ROX normalization (e.g., Agilent, Stratagene).
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Ready-to-use formulation: Simplifies setup and minimizes pipetting errors.
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Fast and efficient cycling: Optimized for rapid thermal cycling with high efficiency.
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Broad dynamic range: Provides accurate quantification across multiple orders of magnitude.
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Reproducible performance: Consistent batch-to-batch quality for research and routine testing.
Specifications
- Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus.
Includes
Includes for 500 reactions:
– 4 x 1.25 mL qMAXSen™ Green qPCR MasterMix 2x (Low ROX)
The MasterMix includes 2x mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, Reference dye & Proprietary Buffer Components.
Download documentation
Datasheet MSDSInstruments CompatibilityApplications
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Gene expression profiling.
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DNA quantification and copy number analysis.
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SNP detection and genotyping.
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High-throughput screening assays.
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qPCR assay development and validation.
Tables & Figures
Quality Control
- Each lot is tested for amplification efficiency, linearity (R² > 0.99), sensitivity, and absence of DNase/RNase contamination.
Advice
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Mix gently before use; avoid vortexing to protect enzyme integrity.
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Always include a no-template control (NTC) to monitor contamination.
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For best results, use high-quality DNA or cDNA templates.
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Keep reagents protected from light to maintain dye stability.
Storage, Shipping & Guarantee
- Shipment at Blue Ice /Gel Pack.
- Storage: -20 °C upon receipt. ROX™ reference dye is sensitive to exposure to light. Avoid repeated freezing and thawing.
Citations
- Janssen, D., González-Miras, E., & Rodríguez, E. (2023). Tuta absoluta-Specific DNA in Domestic and Synanthropic Vertebrate Insectivore Feces. Insects, 14(8), 673.
- Togashi, K., Hatakeyama, S., Yoneyama, T., Hamaya, T., Narita, T., Fujita, N., … & Ohyama, C. (2022). Effect of active anticancer therapy on serologic response to SARS‐CoV‐2 BNT162b2 vaccine in patients with urothelial and renal cell carcinoma. International Journal of Urology.
Safety Statements
This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.
Customers Review
Synonym(s)
SYBR Green qPCR master mix, Real-time PCR master mix SYBR, qPCR Green detection mix, DNA amplification master mix, SYBR Green qPCR mix
Also known as:
- Spanish: Master mix qPCR SYBR Green, Mezcla PCR en tiempo real con SYBR, Master mix qPCR green
- French: Master mix qPCR SYBR Green, Mélange PCR temps réel SYBR, Master mix qPCR green
- German: SYBR Green qPCR Mastermix, Echtzeit-PCR-Mix SYBR, qPCR Green Mastermix
- Italian: Master mix qPCR SYBR Green, Miscela PCR in tempo reale SYBR, Master mix qPCR green
FAQs
Q1: What is the difference between Low ROX and High ROX versions?
Low ROX contains a reduced concentration of passive reference dye, suitable for systems such as Agilent Mx3000P, ABI 7500 Fast, and Stratagene instruments.
Q2: Is the mix compatible with SYBR® Green assays?
Yes. qMAXSen™ Green qPCR MasterMix is fully compatible with SYBR®-based intercalating dye detection.
Q3: Does the mix require additional MgCl₂?
No. The Mg²⁺ concentration is optimized for maximum performance and efficiency.
1 review for qMAXSen™ Green qPCR MasterMix (Low ROX™)
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Tom Hendricks –
We switched to this mastermix specifically because our Agilent instrument requires low ROX normalization, and it works exactly as expected. The ready-to-use 2x format simplifies setup considerably – just add template, primers, and water. Sensitivity is excellent even with low-copy templates, and we get consistent Ct values across runs. Solid batch-to-batch reproducibility, which matters a lot for ongoing expression studies.