Green qPCR MasterMix – Canvax high-sensitivity quantitative PCR mix

qMAXSen™ Green qPCR MasterMix (w/o ROX™)

Name: qMAXSen™ Green qPCR MasterMix (w/o ROX™)
SKU: E0534
Price: 209.00 EUR
Availability: InStock

High-sensitivity 2× master mix for qPCR platforms that do not require ROX reference dye.

qMAXSen™ Green qPCR MasterMix (w/o ROX) from Canvax™ is a ready-to-use, 2× concentrated reaction mix optimized for quantitative real-time PCR (qPCR) using intercalating dye detection systems that do not require ROX normalization.

The advanced formulation combines a hot-start DNA polymerase, optimized buffer, dNTPs, and MgCl₂ to deliver exceptional sensitivity, reproducibility, and linear quantification across a broad dynamic range.
This master mix ensures precise amplification, high fluorescence signal, and consistent results across all qPCR platforms without passive reference dye correction.

Canvax™ qMAXSen™ qPCR Kits has been Nominee as “Best New Life Sciences Product of 2018” at Scientists’ Choice Awards®.

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500 rxn

Price: 209.00 €

SKU: E0534 Categories: qPCR, qPCR MasterMixes

Detailed information:

Advantages & Features

No ROX normalization required: Compatible with instruments not using ROX reference dye (e.g., Bio-Rad, Roche, Illumina).
Exceptional sensitivity: Detects low-copy targets with high signal-to-noise ratio.
Ready-to-use 2× mix: Simplifies setup and reduces pipetting errors.
Fast and efficient cycling: Optimized for short qPCR protocols with high yield and specificity.
Wide dynamic range: Accurate quantification over several orders of magnitude.

Specifications

Taq Source: An E. coli strain with a cloned Taq DNA Polymerase gene from Thermus aquaticus.

Includes

Includes for 500 reactions:
– 4 x 1.25 mL qMAXSen™ Green qPCR MasterMix 2x (w/o ROX)
The MasterMix includes 2x mixture of dNTPs, HotStart DNA Polymerase, Mg2+, fluorescent detection dye, Reference dye & Proprietary Buffer Components.

Download documentation

Datasheet

MSDS

Instruments Compatibility

Applications

Gene expression analysis.
DNA quantification and copy number determination.
SNP genotyping.
High-throughput qPCR assays.
qPCR validation and development workflows.

Tables & Figures

Quality Control

Each production lot is tested for amplification efficiency, specificity, sensitivity, and reproducibility.

Advice

Mix gently before use; avoid vortexing.
Always include a no-template control (NTC).
Store protected from light to preserve fluorescence stability.
Use purified DNA or cDNA free of PCR inhibitors for best results.

Storage, Shipping & Guarantee

Shipment at Blue Ice /Gel Pack.
Storage: -20 °C upon receipt. ROX™ reference dye is sensitive to exposure to light. Avoid repeated freezing and thawing.

Citations

Janssen, D., González-Miras, E., & Rodríguez, E. (2023). Tuta absoluta-Specific DNA in Domestic and Synanthropic Vertebrate Insectivore Feces. Insects, 14(8), 673.
Togashi, K., Hatakeyama, S., Yoneyama, T., Hamaya, T., Narita, T., Fujita, N., … & Ohyama, C. (2022). Effect of active anticancer therapy on serologic response to SARS‐CoV‐2 BNT162b2 vaccine in patients with urothelial and renal cell carcinoma. International Journal of Urology.
Petkova, Mariana, et al. “MICROBIOLOGICAL AND PHYSICOCHEMICAL CHARACTERIZATION OF TRADITIONAL BULGARIAN SOURDOUGHS AND SCREENING OF LACTIC ACID BACTERIA FOR AMYLOLYTIC ACTIVITY.” Journal of Chemical Technology and Metallurgy 55.5 (2020): 921-934.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Customers Review

FAQs

Q1: What is the difference between w/o ROX and Low/High ROX versions?
The w/o ROX formulation contains no passive reference dye, making it ideal for systems that do not require ROX normalization, such as Bio-Rad CFX, Roche LightCycler, or Illumina Eco instruments.

Q2: Is it compatible with SYBR® Green assays?
Yes. qMAXSen™ Green qPCR MasterMix is fully compatible with SYBR®-based detection methods.

Q3: Can it be used for multiplex reactions?
Yes, provided that each target uses primers with similar annealing temperatures and efficiency.