HotBegan™ Hot Start Taq-DNA Polymerase

For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg

HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.

Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.

Price: 70.00

SKU: P0028 Categories: ,

Detailed information:

Advantages & Features
  • High specificity: minimizes unspecific amplification.
  • Efficient: prevents or minimizes primer-dimer and nonspecific products.
  • Great sensitivity: amplifies from femptograms of DNA targets.
  • Inactive: at Room Temperature.
  • Optimized: adds extra nucleotides (preferentially adenine) without template at 3´ends leaving 3´overhangs PCR fragments.
  • Powerful: amplification of targets up to 5 kb.
  • Convenient: available in kits and MasterMix solution.
Specifications

Unit definition:
One unit is defined as the amount of enzyme required to catalyse the incorporation of 10 nanomoles of dNTPs into acid-insoluble material in 30 minutes at 74°C.

Includes

– 100 μL HotBegan™ Hot Start Taq DNA Polymerase (5 U/μL)
– 25 mM MgCl2 (1.5 mL)
– 1.5 mL Buffer (10x)

Download documentation
Applications
  • qPCR.
  • PCR and RT-PCR.
  • Genotyping with Taqman Probes.
  • PCR fragments amplification for TA or GC cloning.
  • Amplification from a limited DNA template or low copy number genes.
Tables & Figures

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Quality Control
  • Functionally tested in qPCR.
  • None detected bacterial DNA (by qPCR).
  • Exempt of nucleases (endo-, exo and ribonucleases) activity guaranteed by appropriate quality tests.
Advice
Storage, Shipping & Guarantee
  • Shipped in: Gel pack.
  • Storage: -20 °C.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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