HotBegan™ Hot Start Taq-DNA Polymerase
For a Specific, Highly Efficient & Reliable Amplifications of DNA up 160 fg
HotBegan™ Hot Start Taq DNA Polymerase is a specific, highly efficient and sensitive HotStart DNA Polymerase designed to minimize unspecific amplification, improving PCR specificity. It is a Horse-Power™ Taq DNA Polymerase bound to a proprietary antibody that blocks polymerase activity until a denaturation step occurs. The heat-labile antibodies are rapidly inactivated by raising the temperature (4 minutes at 95-97 °C). This prevents or minimizes primer-dimer and non-specific products.
Like Horse-Power™ Taq DNA Polymerase, the enzyme has 5’→3’ polymerase activity and a weak 5’→3’ exonuclease activity but no 3’→5’ exonuclease activity (proofreading). Before enzyme activation none of the enzyme activities are detectable.