HigherPurity™ Soil DNA Isolation Kit

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 100 Collection tubes (2 mL)
– 50 Dry bead tube
– 12 g Glass Bead
– 40 mL Lysis Solution 1 (LS1)
– 20 mL WB1 Buffer
– 35 mL EB Buffer
– 15 mL Inhibitor Removal Buffer (IR-Buffer)
– 50 microcentrifuge tube (1.5 mL)
– 15 mL Buffer A
– 25 mL Buffer

Purified DNA suitable for all common Molecular Biology applications, such as:

• PCR and qPCR amplification.
• Restriction enzyme digestion.
•  Automated sequencing.
• Metagenomic and microbial diversity analysis.
• Southern Blots.

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• Tested for isolation of DNA from soil sample.
• The quantity and quality of purified DNA attend to:
  • Ratio 260/ 280 (1.8-2).
  • Agarose gel electrophoresis.

• Precautions: Please wear gloves when using this product. Avoid all skin contact with kit reagents. In case of contact, wash thoroughly with water.

• Shipped in: Ambient Temperature.
• Storage: All components can be stored at Room Temperature, except Proteinase K (4 ºC). Proteinase K should be stored at -20 ºC for longer-term storage.

• Pastor Llamas, I. (2026). Bioprospección De Bacterias Del Suelo Productoras De Compuestos Antimicrobianos Con Potencial Aplicación En La Bioconservación De La Leche. 
• Pavlovska, M., Prekrasna-Kviatkovska, Y., Zotov, A., Dzhulai, A., Dykyi, E., Huettel, B., … & Sidhu, C. (2026). Phytoplankton dynamics shape bacterioplankton community structure and metabolism during the austral summer-autumn transition in the Western Antarctic Peninsula. FEMS Microbiology Ecology, fiag053.
• Allegue, L. D., Ventura, M., Vlaeminck, S. E., Melero, J. A., Leroy, B., & Puyol, D. (2026). Purple phototrophic bacteria release crotonate as a metabolic overflow pathway. Communications Biology.
• Martínez-Subirá, M., Alfonso, M. E. C., Friero, I., Macià, A., Pena, R. N., Molinero, N., … & Moralejo, M. (2026). Barley extrudates modulate the gut microbiome–metabolome axis in vitro through β-glucan fermentation and polyphenol biotransformation. Food & Function.
• Puyol, D., Allegue, L., Ventura, M., Vlaeminck, S., Melero, J., & Leroy, B. (2025). Purple phototrophic bacteria release crotonate as metabolic overflow pathway to complement other redox-balancing routes.
• Sáez‐Sandino, T., Reich, P. B., Maestre, F. T., Cano‐Díaz, C., Stefanski, A., Bermudez, R., … & Trivedi, P. (2025). A Large Fraction of Soil Microbial Taxa Is Sensitive to Experimental Warming. Global Change Biology, 31(5), e70231.
• Cortijo-Alfonso, M. E., Laghouaouta, H., Pena, R. N., Martínez-Subirà, M., Yuste, S., Rubió-Piqué, L., & Piñol, C. (2025). Gut Microbiota Modulation And Inflammation Mitigation In a Murine Model Through A Hull-Less And Purple Grain Barley Genotype. Food & Function.
• Azouggagh, L., Ibáñez-Escriche, N., Martínez-Álvaro, M., Varona, L., Casellas, J., Negro, S., & Casto-Rebollo, C. (2025). Characterization of microbiota signatures in Iberian pig strains using machine learning algorithms. Animal Microbiome, 7(1), 13.
• Withey, Z., & Gweon, H. S. (2024). Longitudinal bacterial community dynamics and sodium hypochlorite intervention in a newly built university building. Science of The Total Environment, 175349.
• Withey, Z., Awan, A., Duguma, N., Fell, E., Martinez, N. J., Neary, E., … & Gweon, H. S. (2023). Mycobial community assemblages in sink drains across a university campus. Environmental DNA, 5(1), 212-224.
• Prasedya, E. S., Kurniawan, N. S. H., Ardiana, N., Ilhami, B. T. K., Mulyaningsih, T., Astuti, S. P., … & Widyastuti, S. (2022). Effects of Fermented Seaweed Fertilizer Treatment on Paddy Amino Acid Content and Rhizosphere Microbiome Community. Fermentation, 8(9), 420.
• Prasedya, E. S., Kurniawan, N. S. H., Kirana, I. A. P., Ardiana, N., Abidin, A. S., Ilhami, B. T. K., … & Nikmatullah, A. (2022). Seaweed Fertilizer Prepared by EM-Fermentation Increases Abundance of Beneficial Soil Microbiome in Paddy (Oryza sativa L.) during Vegetative Stage. Fermentation, 8(2), 46.
• Del Álamo, A. C., Pariente, M. I., Molina, R., & Martínez, F. (2022). Advanced bio-oxidation of fungal mixed cultures immobilized on rotating biological contactors for the removal of pharmaceutical micropollutants in a real hospital wastewater. Journal of Hazardous Materials, 425, 128002.
• Withey, Zoe, et al. “Characterization of communal sink drain communities of a university campus.” Environmental DNA (2021).
• Семенов, М. В. (2019). Метабаркодинг и метагеномика в почвенно-экологических исследованиях: успехи, проблемы и возможности. Журнал общей биологии, 80(6), 403-417.
• Kononenko, N., Baranova, E., Dilovarova, T., Akanov, E., & Fedoreyeva, L. (2020). Oxidative Damage to Various Root and Shoot Tissues of Durum and Soft Wheat Seedlings during Salinity. Agriculture, 10(3), 55.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Soil DNA extraction kit, Environmental DNA extraction soil, Soil DNA purification kit, DNA isolation soil samples, Soil genomic DNA kit

Also known as:

• Spanish: Kit de extracción de ADN de suelo, Aislamiento de ADN ambiental, Purificación de ADN de suelo
• French: Kit d’extraction ADN sol, Isolation ADN environnemental, Purification ADN sol
• German: DNA-Extraktionskit für Bodenproben, Umwelt-DNA-Isolation, DNA-Reinigung Boden
• Italian: Kit estrazione DNA suolo, Isolamento DNA ambientale, Purificazione DNA suolo

Q: Are the bead tubes supplied pre-filled?
A: Yes, bead tubes are pre-filled with glass beads and ready to use.

Q: Is a bead-beating homogenizer required?
A: No, the protocol uses vortex-based homogenization and does not require specialized equipment.