WideUSE™ Plasmid Maxiprep Kit

Name: WideUSE™ Plasmid Maxiprep Kit
SKU: AN0169
Price: 313.52 EUR
Availability: InStock

Fast and reliable Maxiprep kit for high-quality plasmid DNA purification using anion-exchange technology — transfection-grade purity in minutes.

WideUse™ Plasmid Maxiprep Kit provides a fast, simple, and highly efficient method for the routine isolation of high-quality plasmid DNA in Maxiprep format.
Based on anion-exchange column technology, this kit ensures high yield and purity suitable for all downstream molecular biology applications, including transfection-grade plasmid DNA.

The kit incorporates an innovative Lysis Control Reagent (LCR), a color indicator that enables visual identification of optimal buffer mixing. This prevents common errors such as incomplete cell lysis or poor SDS/genomic DNA precipitation, ensuring reproducibility and maximum yield even for less experienced users.

Whether for routine plasmid preparations or large-scale molecular workflows, the WideUse™ Maxiprep Kit delivers consistent, high-quality DNA ready for enzymatic reactions, sequencing, or transfection experiments.

Have questions? Contact us

10 rxn

Price: 313.52 €

SKU: AN0169 Categories: DNA Extraction Kits, DNA & RNA Extraction

Detailed information:

Advantages & Features

High-quality plasmid DNA: Suitable for all molecular biology applications.
Transfection-grade purity: Reliable performance in sensitive assays.
Fast & simple: Obtain results in just minutes.
Safe: No phenol–chloroform extraction required.
Complete solution: Includes optional filters to remove cellular debris.
Visual lysis control: Lysis Control Reagent (LCR) ensures optimal buffer mixing.
Maxiprep format: Ideal for high-yield preparations.
Anion-exchange technology: Ensures reproducibility and purity.

Specifications

Includes

Includes for 10 rxn:
– 110 mL Resuspension Solution
– 150 mL Lysis Solution (BLY)
– 110 mL Neutralization Solution
– 130 mL Equilibration Buffer
– 360 mL Wash Buffer (WB1)
– 130 mL Elution Buffer (EB)
– 1,500 µL Lysis control reagent (LCR)
– 25 mL TE Buffer
– 10 Plasmid Midi / Maxi Columns
– 200 µL RNase (50 mg/mL)
– 10 Folder Filters

Download documentation

Datasheet

MSDS

Applications

Purified plasmid DNA is ready for use in a broad range of molecular biology applications, including:

Digestion with restriction enzymes.
Automated sequencing.
PCR template.
Bacterial transformation.
Transfection.

Tables & Figures

Quality Control

– Tested for the isolation of any Plasmid DNA from transformed E.coli.
– The quality of purified DNA is analysed by:

Ratio 260/ 280.
Agarose gel electrophoresis.
Digestion with restriction endonucleases.

Advice

Use gloves and standard laboratory protection while handling reagents.
Avoid contamination with genomic DNA by ensuring complete cell lysis and neutralization.

Storage, Shipping & Guarantee

Shipped in: Ambient temperature.
Storage: All components should be stored at room temperature (15–25 °C) for up to 12 months without any reduction in performance. Store the RNAse A at -20 ºC. After addition of RNase A to Resuspension Solution can be stored at 4 ºC. Verify that the Lysis Solution (BLY) does not contain precipitated SDS due to the low temperatures. If necessary, dissolve the SDS heating at 37 °C.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.