WideUSE™ Plasmid Purification MiniSpin Kit

Name: WideUSE™ Plasmid Purification MiniSpin Kit
SKU: AN0068
Availability: InStock

High-yield plasmid DNA isolation in ~30 minutes using WideUse™ silica MiniSpin technology.

The WideUse™ Plasmid Purification MiniSpin Kit provides a fast, reliable and cost-effective method for isolating high-quality plasmid DNA from E. coli. Based on advanced silica-membrane technology, the kit ensures reproducible yields of highly pure DNA free of proteins, RNA and other contaminants.

The extracted plasmid DNA is immediately ready for use in a wide range of downstream molecular biology applications, such as restriction digestion, PCR, qPCR, sequencing or transfection. Its optimized buffers and high-capacity columns guarantee consistent results even with challenging samples.

Designed for routine plasmid minipreps in research laboratories, the WideUse™ kit combines simplicity, reproducibility and superior performance at an affordable price.

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Price: 77.84 € – 253.23 €

size

50 rxn

100 rxn

250 rxn

SKU: AN0068 Categories: DNA Extraction Kits, DNA & RNA Extraction

Detailed information:

Advantages & Features

Highly efficient: yields up to 24 µg (depending on plasmid copy number, culture volume and bacterial growth conditions).
Broad range: optimized for plasmid sizes between 1 and 15 kb.
Pure plasmid DNA: ready-to-use in all Molecular Biology applications.
Swift and easy procedure: obtain plasmid DNA in ~25–30 minutes with minimal handling.
Safe: avoids phenol/chloroform extraction.
Convenient MiniSpin format: compatible with standard centrifuge
Cost-effective solution for routine plasmid preps.

Specifications

Includes

Includes for 50 rxn:
– 50 CleanEasy™ MiniSpin Columns
– 50 Collection tubes
– 16 mL S-I Buffer
– 16 mL S-II Buffer
– 16 mL S-III Buffer
– 30 mL Binding Buffer
– 8 mL Washing Buffer
– 10 mL Elution Buffer
– 160 μL RNAse

Download documentation

Datasheet

MSDS

Applications

Purified DNA suitable for all common Molecular Biology applications, such as:

Restriction digestion and cloning
Routine plasmid DNA preparation from E. coli
Automated sequencing.
PCR template.
Transfection.
Library construction & screening

Tables & Figures

Quality Control

Tested for the isolation of any plasmid DNA from transformed E.coli.
The Quality of Purified DNA is analysed by:
- Ratio 260/ 280 (1.8-2).
- Agarose Gel Electrophoresis.
- Digestion with Restriction Endonucleases.

Advice

Use fresh overnight cultures in exponential phase for maximum yields.
Do not overload columns to ensure purity.
Equilibrate buffers to room temperature before starting the procedure

Storage, Shipping & Guarantee

Shipped in: Ambient Temperature.
Storage: All components can be stored at Room Temperature (15–25 °C) , except RNAse A (-20 ºC).
Recommendations: After addition of RNase A to Buffer S-I reagent can be stored at 4 ºC.

Citations

Gómez-Melero, S. (2022). Desarrollo de anticuerpos terapéuticos contra el receptor CCR6 humano.
Veva, A. M. M. (2021). Production of T. gondii transgenic strains to characterize the tubulin cofactor B interactome (Doctoral dissertation, Instituto Politécnico de Lisboa, Escola Superior de Tecnologia da Saúde de Lisboa).
Jiménez Muñoz, R. (2020). Desarrollo de herramientas fisiológicas y genómicas para mejorar la calidad postcosecha del fruto de calabacín.
Maza-Márquez, P., Aranda, E., González-López, J., & Rodelas, B. (2020). Evaluation of the Abundance of Fungi in Wastewater Treatment Plants Using Quantitative PCR (qPCR). In Quantitative Real-Time PCR (pp. 79-94). Humana, New York, NY.
Maza-Márquez, P., Castellano-Hinojosa, A., González-Martínez, A., Juárez-Jiménez, B., González-López, J., & Rodelas, B. (2019). Abundance of total and metabolically active Candidatus Microthrix and fungal populations in three full-scale wastewater treatment plants. Chemosphere.

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.