WideUSE™ Plasmid Midiprep Kit

Name: WideUSE™ Plasmid Midiprep Kit
SKU: AN0168
Price: 313.52 EUR
Availability: InStock

Reliable anion-exchange Midiprep kit for fast and high-quality plasmid DNA purification — transfection-grade DNA in minutes.

WideUse™ Plasmid Midiprep Kit provides a fast, simple, and highly efficient method for the routine isolation of high-quality plasmid DNA in Midiprep format.
Based on anion-exchange column technology, this kit delivers reproducible, transfection-grade plasmid DNA suitable for all downstream molecular biology applications.

The kit incorporates a Lysis Control Reagent (LCR), a visual color indicator that enables users to identify optimal buffer mixing during cell lysis.
This innovation minimizes user errors such as incomplete lysis or precipitation, ensuring consistent DNA quality and yield.

Ideal for both beginners and experienced researchers, the WideUse™ Plasmid Midiprep Kit offers reliable performance, scalability, and superior purity — perfect for any standard molecular biology workflow.

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25 rxn

Price: 313.52 €

SKU: AN0168 Categories: DNA Extraction Kits, DNA & RNA Extraction

Detailed information:

Advantages & Features

High-quality plasmid DNA: Ready-to-use for all molecular biology applications.
Transfection-grade purity: Ideal for sensitive downstream assays.
Fast & simple: Obtain high-purity plasmid DNA in minutes.
Safe: No phenol–chloroform extraction required.
Complete solution: Includes specialized filters for debris removal.
- Visual lysis control: LCR reagent ensures optimal buffer mixing.
Midiprep format: Suitable for medium-scale plasmid preparation.
Anion-exchange technology: Ensures reproducibility and consistent yields.

Specifications

Includes

Includes for 25 rxn:
– 110 mL Resuspension Solution
– 150 mL Lysis Solution (BLY)
– 110 mL Neutralization Solution
– 130 mL Equilibration Buffer
– 360 mL Wash Buffer (WB1)
– 220 mL Elution Buffer (EB)
– 1,500 µL Lysis control reagent (LCR)
– 25 mL TE Buffer
– 25 Plasmid Midi / Maxi Columns
– 200 µL RNase (50 mg/mL)
– 25 Folder Filters

Download documentation

Datasheet

MSDS

Applications

All Molecular Biology applications, such as:

Digestion with restriction enzymes.
Automated sequencing.
PCR template.
Bacterial transformation.
Transfection.

Tables & Figures

Quality Control

– Tested for the isolation of any plasmid DNA from transformed E.coli.
– The quality of purified DNA is analysed by:

Ratio 260/ 280.
Agarose gel electrophoresis.
Digestion with restriction endonucleases.

Advice

Wear gloves and protective clothing while handling reagents.
Ensure complete cell lysis and neutralization to avoid genomic DNA contamination.

Storage, Shipping & Guarantee

Shipped in: Ambient temperature.
Storage: All components should be stored at room temperature (15–25 °C) for up to 12 months without any reduction in performance. Store the RNAse A at -20 ºC. After addition of RNase A to Resuspension Solution can be stored at 4 ºC. Verify that the Lysis Solution (BLY) does not contain precipitated SDS due to the low temperatures. If necessary, dissolve the SDS heating at 37 °C.

Citations

Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.