CIAP (Calf Intestinal Alkaline Phosphatase)

Unit Definition
One unit is defined as the amount of enzyme that hydrolyzes 1 μmol of p-Nitrophenyl Phosphate (PNPP) in a total reaction volume of 1 ml in 1 minute, at 37 °C.

– Alkaline Phosphatase (CIAP)
– Alkaline Phosphatase Buffer (10x)

• DNA and RNA dephosphorylation.

• Vector self-ligation prevention.

• Labeling and end-modification reactions.

• Protein dephosphorylation studies.

• Each batch is tested for enzyme activity, purity (SDS-PAGE), and absence of endonuclease contamination.

• Use 0.01–1 U of CIAP per µg of DNA.

• Inactivate by heating at 65 °C for 10 minutes.

• Avoid repeated freeze–thaw cycles; aliquot if needed.

• Shipped in: Gel pack.
• Storage: -20 °C.

• López, C. M., Alseekh, S., Martínez Rivas, F. J., Fernie, A. R., Prieto, P., & Alamillo, J. M. (2024). CRISPR/Cas9 editing of two adenine phosphoribosyl transferase coding genes reveals the functional specialization of adenine salvage proteins in common bean. Journal of Experimental Botany, erae424.
• López Vázquez, C. (2023). Effects of symbiotic N fixation on N and water use efficiency in common bean plants: physiological, molecular and biochemical characterization.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What is the main function of CIAP?
It removes 5′-phosphate groups to prevent vector self-ligation during cloning.

Q2: Can it be used for RNA and protein substrates?
Yes, CIAP dephosphorylates DNA, RNA, and phosphoproteins efficiently.

Q3: How is CIAP inactivated after use?
By heating at 65 °C for 10 minutes or by phenol extraction.