PRImeDETECT™ Salmonella Detection Kit

• Amplification limit: 10 UG per reaction (100%), 2 UG per reaction (40%).
• Quantification limit:  20 UG per reaction (100%).
• Quantification Dynamic range: 6 logs.
• Target: Salmonella spp.
• Inclusivity: 100% Inclusivity.  700 Salmonella strains tested, including S.enterica and S. bongori
• Exclusivity:  100%.  Tested with 42 strains of the rest of Enterobacteriaceae and Eubacteria
• Detection:Probe labelled with fluorescent dyes Salmonella spp: Cy5-BHQ1; IAC: HEX-BHQ1.
• Thermal cCycler: Agilent Mx3005P, Applied Biosystems 7300, 7500 and other cyclers.

– 2 x PCR MasterMix (48 rxn each)
– 2 x DNAready Lysis Buffer (48 rxn each)
– 1 x PCR Positive Control
– 1 x PCR Negative Control

• Salmonella detection in food samples.

• Water and environmental monitoring.

• Food safety compliance testing.

• Research on Salmonella contamination.

• Always include both positive and negative controls in each qPCR run.

• Use proper sample preparation to minimize PCR inhibition.

• Store reagents in aliquots to avoid freeze-thaw degradation.

• Shipment at Ambient Temperature.
• Storage: -20 °C upon receipt. Avoid prolonged exposure to light. If stored correctly the kit will retain full activity for 12 months. The kit can be stored at 4°C for 1 month.

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

Q1: What type of samples can be tested with this kit?
It is validated for food, water, and environmental samples.

Q2: How does this kit compare to culture methods?
It provides results within hours, while culture methods can take several days.

Q3: Are internal controls included?
Yes, positive and negative controls are included to ensure reliable results.