RNAse Inhibitor

Recombinant Protein which specifically inhibits Ribonucleases A, B and C (40 U/μL)

RNase Inhibitor is a recombinant protein which specifically inhibits Ribonucleases A, B and C. The protein inhibits RNases by binding noncovalently in a 1:1 ratio with a potent affinity (ki >10-14 M). RNAse inhibitor protein is purified from E. coli strain harbouring a plasmid with cloned gene coding of mammalian RNase inhibitor.

GMP-grade reagent also available. Learn more.

Price: 264.00 

SKU: P0270 Categories: ,

Detailed information:

Advantages & Features
  • Reliable: stringent quality control standards to guarantee lot-lot consistency.
  • High Quality: free of DNA exo- and endonuclease, and RNase contamination.
  • Proven performance: for described applications.
Specifications

Unit Definition
1 U of RNAse inhibitor is defined as an amount of enzyme that blocks activity of 5 ng of RNase A in 50%.

Stability

The enzyme is stable up to 58°C and DTT concentration ranges ( 0.5–1 mM DTT presence is essential for optimal activity). Inactivation of the enzyme occurs at 65°C. The enzyme is active over a broad pH range (pH 5.5 – 9.0).
Includes

Includes for 10,000 U:
– 10,000 U RNAse Inhibitor (40 U/μL)

Download documentation
Datasheet
MSDS
Applications

– Inhibition of RNA degradation in a variety of Molecular Biology applications:

  • cDNA synthesis.
  • RT-PCR.
  • In vitro transcription/translation systems.
  • Isolataion and storage of RNA.
  • In vitro RNA synthesis.
Tables & Figures

Quality Control
  • Free of detectable DNA exo- and endonuclease, and RNase activities.
  • Strict quality controls in every phase of manufacturing to guarantee the highest quality and reproducibility.
Advice
Storage, Shipping & Guarantee
  • Shipped in: Gel pack.
  • Storage: -20 °C.
Citations
Safety Statements

This product is developed, designed and sold exclusively for Research purposes and in vitro use only (RUO). The product was not tested for use in diagnostics or for drug development, nor is it suitable for administration to humans or animals. For more info, please check its Material Safety Data Sheet available in this website.

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